DNA isolation from teeth by organic extraction and identification of sex of the individual by analyzing the AMEL gene marker using PCR.

Background: To identify the sex of the deceased individual from dental hard tissue such as enamel and dentine.

Objective: To isolate the DNA from dental hard tissue (enamel and dentin) from teeth extracted for prophylactic purpose, to assess the quality and purity of DNA and to identify the sex using polymerized chain reactor (PCR).

Materials And Methods: DNA was extracted following phenol/chloroform (organic) extraction from 20 male and 20 female teeth.