Publications by authors named "Subramanian Saravanan"

Article Synopsis
  • Sepsis, a severe condition causing many deaths worldwide, requires effective biomarkers for predicting its progression to septic shock, which current methods have struggled to provide.
  • Researchers developed a machine learning model named SepxFindeR using data from several transcriptomics datasets to better distinguish between sepsis and septic shock.
  • The model identified six key genes related to immune response, demonstrating high accuracy in predicting disease stages, and allows for effective patient identification using blood samples.
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Microbiota and feeding modes influence the susceptibility of premature newborns to necrotizing enterocolitis (NEC) through mechanisms that remain unknown. Here, we show that microbiota colonization facilitated by breastmilk feeding promotes NOD-like receptor family CARD domain containing 5 (Nlrc5) gene expression in mouse intestinal epithelial cells (IECs). Notably, inducible knockout of the Nlrc5 gene in IECs predisposes neonatal mice to NEC-like injury in the small intestine upon viral inflammation in an NK1.

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The pathogenic outcome of enteric virus infections is governed by a complex interplay between the virus, intestinal microbiota, and host immune factors, with metabolites serving as a key mediator. Noroviruses bind bile acid metabolites, which are produced by the host and then modified by commensal bacteria. Paradoxically, bile acids can have both proviral and antiviral roles during norovirus infections.

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Apoptosis, inflammation, and wound healing are critical pathophysiological events associated with various liver diseases. Currently, there is a lack of in vivo approaches to study hepatocyte apoptosis-induced liver injury and repair. To address this critical knowledge gap, we developed a unique genetically modified mouse model, namely, 3-Transgene (Tg) with inducible Hepatocyte-Specific Apoptosis Phenotype (3xTg-iHAP) in this study.

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Background & Aims: Necrotizing enterocolitis (NEC) is a life-threatening disease affecting mostly the ileum of preemies. Intestinal epithelial cell (IEC) apoptosis contributes to NEC pathogenesis. However, how scattered crypt IEC apoptosis leads to NEC with excessive villus epithelial necrosis remains unclear.

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Article Synopsis
  • Noroviruses cause 685 million cases of acute gastroenteritis worldwide each year, significantly impacting children, with around 200 million pediatric cases and up to 200,000 deaths.
  • Children are more susceptible to severe infections due to the unique bile acid pool in newborns, influenced by their developing gut microbiota and immature metabolic pathways.
  • Research shows that microbiota-derived bile acids can protect infants from severe norovirus symptoms, while maternal bile acids can increase vulnerability; hence, targeting bile acid metabolism may reduce neonatal infection risk.
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Fluoride is widely present in nature, and human exposure to it is generally regarded as inevitable. High levels of fluoride intake induce acute and chronic illnesses. To reduce potential harm to the general public, it is essential to create selective fluoride detectors capable of providing a colorimetric response for naked-eye detection without the need for sophisticated equipment.

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Clinical evidence indicates a connection between gut injuries, infections, inflammation, and an increased susceptibility to systemic inflammation. Nevertheless, the animal models designed to replicate this progression are inadequate, and the fundamental mechanisms are still largely unknown. This research explores the relationship between gut injuries and systemic inflammation using a Dextran Sulfate Sodium (DSS)-induced colonic mucosal injury mouse model.

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Metallogels formed from supramolecular interactions of low-molecular-weight gelators (LMWGs) combine the qualities of heterogeneous catalysts and offer the advantages of multifunctionality owing to the facile installation of desired task-specific moieties on the surface and along the channels of the gels. We discuss the applications of a triazole-based Ni(II) gel-derived xerogel () having a high density of Ni(II)-nodes and appended primary amines as a recyclable heterogeneous catalyst for Knoevenagel condensation of aldehyde and malononitrile in water and the solvent-free cycloaddition of CO to form a series of cyclic carbonates with near-quantitative conversion of the respective epoxides, with low catalyst loading (0.59 mol %), high catalyst stability, and recyclability.

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Introduction The knee joint is a complex system containing various hard and soft tissue components necessary for functioning in a coordinated manner. The menisci help to deepen the tibial plateau. Knowledge of the dimension of menisci in the knee joint is of paramount importance in arthroscopic surgery and the management of injuries due to sports or degeneration.

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In this study, we determined if B lymphocytosis may serve as a JDM biomarker for disease activity. Children with untreated JDM were divided into two groups based on age-adjusted B cell percentage (determined through flow cytometry): 90 JDM in the normal B cell group and 45 in the high B cell group. We compared through -testing the age, sex, ethnicity, duration of untreated disease (DUD), disease activity scores for skin (sDAS), muscle (mDAS), total (tDAS), CMAS, and neopterin between these two groups.

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Background: Saliva has the potential to be used as a noninvasive sample for testing hyperglycemia in diabetes mellitus. Serum 1,5-anhydroglucitol (1,5-AG) decreases with an increase in blood sugar >180 mg/dl. We hypothesized that salivary 1,5-AG can be used to identify blood sugar higher than 180 mg/dl using a novel biochemical method.

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One of the fundamental aims in catalysis research is to understand what makes a certain scaffold perform better as a catalyst than another. For instance, in nature enzymes act as versatile catalysts, providing a starting point for researchers to understand how to achieve superior performance by positioning the substrate close to the catalyst using non-covalent interactions. However, translating this information to a non-biological catalyst is a challenging task.

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Feeding modes influence the gut microbiome, immune system, and intestinal barrier homeostasis in neonates; how feeding modes impact susceptibility to neonatal gastrointestinal (GI) diseases is still uncertain. Here, we investigated the impact of dam feeding (DF) and formula feeding (FF) on features of the gut microbiome and physiological inflammation during the first 2 days of postnatal development and on the susceptibility to intestinal injury related to the inflammatory state in neonatal mouse pups. 16S rRNA sequencing data revealed microbiome changes, lower α-diversity, and a distinct pattern of β-diversity including expansion of and in the ileum of FF pups compared with DF pups by postnatal day (P)2.

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Complement component 1 Q subcomponent binding protein (C1QBP) plays a vital role in the progression and metabolism of cancer. Studies have shown that xanthine dehydrogenase (XDH)-derived reactive oxygen species (ROS) accelerates tumor growth, and also induces mutations or produces cytotoxic effects concurrently. However, the role of C1QBP in metabolism, oxidative stress, and apoptosis of renal cell carcinoma (RCC) cells have not yet been explored.

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Controlling the product selectivity of a ring-opening hydrolysis reaction remains a great challenge with mineral acids and to an extent with homogeneous catalysts. In addition, even trace amounts of metal impurities in a bioactive product hinder the reaction progress. This has necessitated the development of robust and metal-free catalysts to offer an alternative sustainable route.

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Among urological tumors, renal cell carcinoma (RCC) is the third-highest mortality rate tumor, and 20%-30% of RCC patients present with metastases at the time of diagnosis. While the treatment of RCC has been improved over the last few years, its mortality stays high. Y-box binding protein 1 (YBX1) is a well-known oncoprotein that has tumor-promoting functions.

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Infrared thermography provides functional imaging by picturing the temperature pattern of the region imaged. The temperature correlates to the blood flow pattern and is used in the diagnosis of diseases like breast cancer, peripheral vascular disorders, diabetic neuropathy and fever screening. In the present study, the usage of ocular thermography for diagnosis of diabetic retinopathy is explored.

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Infection with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes COVID-19, a disease that involves significant lung tissue damage. How SARS-CoV-2 infection leads to lung injury remains elusive. The open reading frame 8 (ORF8) protein of SARS-CoV-2 (ORF8) is a unique accessory protein, yet little is known about its cellular function.

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Milk fat globule-EGF factor 8 (MFG-E8) is a secreted glycoprotein that regulates tissue homeostasis, possesses potent anti-inflammatory properties, and protects against tissue injury. The human pancreas expresses MFG-E8; however, the role of MFG-E8 in the pancreas remains unclear. We examined the expression of MFG-E8 in the pancreas at baseline and during cerulein-induced acute pancreatitis in mice and determined whether MFG-E8 attenuates the progression of pancreatitis, a serious inflammatory condition that can be life-threatening.

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Stoichiometric carbon dioxide reduction to highly reduced C1 molecules, such as formic acid (2e ), formaldehyde (4e ), methanol (6e ) or even most-reduced methane (8e ), has been successfully achieved by using organosilanes, organoboranes, and frustrated Lewis Pairs (FLPs) in the presence of suitable catalyst. The development of renewable organohydride compounds could be the best alternative in this regard as they have shown promise for the transfer of hydride directly to CO . Reduction of CO by two electrons and two protons to afford formic acid by using renewable organohydride molecules has recently been investigated by various groups.

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Isolated pleural effusion is a rare manifestation of filariasis that mimics tuberculosis, especially in endemic regions. We describe a case of lymphocytic and exudative pleural effusion showing microfilaria on pleural fluid cytology. A retrospective review of all cases of filarial pleural effusion reported after 2000 was conducted to evaluate the association between filariasis and pleural effusion as well as to screen the features that can help in accurate detection of these patients.

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Inflammatory bowel disease (IBD) is a devastating relapsing-remitting systemic disease of the gastrointestinal system. It is characterized by an extremely imbalanced immune system and inflammatory mediators that result from the disruption of a complex series of interactions between the microbiome, the mucosal barrier, and the immune system. Over the past decades, numerous animal models such as chemical, genetic and bacterial models have populated to understanding pathophysiology of IBD and preclinical screening of therapeutic compounds for novel treatments.

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The construction of sophisticated molecular architectures from chemical subunits requires careful selection of the spacers, precise synthetic strategies, and substantial efforts. Here, we report a series of binuclear double-stranded helicates synthesized from different combinations of pyridyl hydrazone-based multidentate ligands (H , H , H ) by increasing the methylene spacer and transition metals (Co, Ni, and Zn). The ligands H ('1,'3-bis(()-pyridin-2-ylmethylene)malonohydrazide), H ('1,'4-bis(()-pyridin-2-ylmethylene)succinohydrazide), and H ('1,'5-bis(()-pyridin-2-ylmethylene)glutarohydrazide) and their respective complexes with Co, Ni, and Zn were obtained.

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Gut injury continues to be the devastating and unpredictable critical illness associated with increased cell death of intestinal epithelial cells (IECs). The IECs, immune system and microbiome are the interrelated entities to maintain normal intestinal homeostasis and barrier integrity. In response to microbial invasion, IEC cell death occurs to maintain intestinal epithelium function and retain the continuous renewal and tissue homeostasis.

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