Ethyl acetate, acetone, 2-propanol, 1-propanol, and ethanol were screened among the class 3 category solvents as an alternative to hexane based on operational and occupational safety and bio-renewability potential. All five solvents exhibited higher extractability (22.3 to 23.
View Article and Find Full Text PDFNonpolar solvents have been reported to exhibit a nonlinear flux-pressure behavior in hydrophobic membranes. This study explored the flux-pressure relationship of six nonpolar solvents in a lab-cast hydrophobic poly(dimethylsiloxane) (PDMS) membrane and integrated the permeance behavior in the evaluation of the proposed transport model. The solvents exhibited a nonlinear relationship with the applied pressure, along with the point of permeance transition (1.
View Article and Find Full Text PDFCompr Rev Food Sci Food Saf
September 2021
Vegetable oil processing has been identified as one of the potential nonaqueous applications of membrane technology. Membrane-based processing has been largely attempted on individual steps of the conventional refining process with reasonable success. With the advent of organic-solvent-nanofiltration, membrane desolventizing of hexane oil miscella has received greater attention, revitalizing the prospects of integrated membrane processing.
View Article and Find Full Text PDFA techno-economic analysis of submerged (SmF) and solid state fermentation (SSF) processes for Aspergillus carbonarius polygalacturonase production was performed to make an appropriate process selection. The downstream processing involved integrated membrane process (IMP) and alginate affinity precipitation (AAP). For a production scale of 30kL purified polygalacturonase concentrate per year, the total upstream cost of SmF was 14% lower than the SSF process.
View Article and Find Full Text PDFAspergillus carbonarius, when grown by submerged and solid-state fermentation, produces different molecular forms of polygalacturonase (PG; EC 3.2.1.
View Article and Find Full Text PDFPurification of polygalacturonase (PG) from the cultures of Aspergillus carbonarius obtained by acetate buffer extraction after solid-state fermentation was attempted by integrated membrane process and alginate affinity precipitation. The carbohydrates were completely eliminated (98%-99%) with a PG recovery of 72%-80% during integrated membrane process, which would otherwise interfere with the purification process and lead to enzyme loss. However, specific activity of PG did not improve (1.
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