Chromatin regulates expression of small RNAs to help maintain transposon methylome homeostasis in Arabidopsis.

Background: Eukaryotic genomes are partitioned into euchromatic and heterochromatic domains to regulate gene expression and other fundamental cellular processes. However, chromatin is dynamic during growth and development and must be properly re-established after its decondensation. Small interfering RNAs (siRNAs) promote heterochromatin formation, but little is known about how chromatin regulates siRNA expression.

Improved shoot regeneration from root explants using an abscisic Acid-containing medium.

Arabidopsis thaliana is an excellent experimental system to study various aspects of plant biology. An efficient regeneration protocol of the plant is important to produce in vitro fertile plants, as well as a fundamental step to study the cellular, physiological, and molecular mechanisms of plant development. Here, we describe an efficient and fast regeneration system from root explants of A.