Correlation of Day 8 Steroid Response with Bone Marrow Status Measured on Days 14 and 35, in Patients with Acute Lymphoblastic Leukemia Being Treated with BFM Protocol.

Berlin-Frankfurt-Munster (BFM) protocol is commonly used in India for treatment of acute lymphoblastic leukemia (ALL). The present study was conducted to correlate day 8 steroid response with bone marrow status (by morphology and flow cytometry) at day 14 and day 35 of treatment. It was a prospective study which included all newly diagnosed ALL patients who visited hospital between March 2013 and February 2015 i.

A new species of Gomphidia Selys, 1854 (Insecta: Odonata: Anisoptera: Gomphidae) from the Western Ghats of India.

A new species of Gomphidia Selys, 1854 is described from southern Western Ghats, India based on a male specimen. The new species is very distinct from all the known species of Gomphidia from Indian subcontinent. Revised keys to the males and known females of Gomphidia of India and Sri Lanka are provided.

A new species of Cyclogomphus Selys, 1854 (Insecta: Odonata: Gomphidae) from the Western Ghats, India with comments on the status of Cyclogomphus vesiculosus Selys, 1873.

A new species of Cyclogomphus Selys, 1854 is described from the Western Ghats of India based on male and female specimens collected from Goa and Kerala states. The new species differs from all the known species of Cyclogomphus in the shape of the anal appendages as well as the thoracic and abdominal markings. We also provide here an updated key to the Cyclogomphus species and comment on the status of Cyclogomphus vesiculosus Selys, 1873.

A new record of the family Isonychiidae (Insecta: Ephemeroptera) from the Western Ghats, India with a description of new species.

A new species belonging to the subgenus Isonychia, of the genus Isonychia Eaton, 1871, is described based on larvae and imagoes collected from Moyar River, Nilgiri District, Tamil Nadu, India. The imagoes of I. moyarensis n.

Quantitating the epigenetic transformation contributing to cholesterol homeostasis using Gaussian process.

To understand the impact of epigenetics on human misfolding disease, we apply Gaussian-process regression (GPR) based machine learning (ML) (GPR-ML) through variation spatial profiling (VSP). VSP generates population-based matrices describing the spatial covariance (SCV) relationships that link genetic diversity to fitness of the individual in response to histone deacetylases inhibitors (HDACi). Niemann-Pick C1 (NPC1) is a Mendelian disorder caused by >300 variants in the NPC1 gene that disrupt cholesterol homeostasis leading to the rapid onset and progression of neurodegenerative disease.

Dislodged Nephrostomy Catheter: An Unusual Case of Idiopathic Transposition of Nephrostomy Catheter From Transplanted Kidney to Adjacent Small Bowel Loop: A Case Report.

A 72-year-old man with a past medical history notable for deceased renal transplant presented to the interventional radiology department for routine right lower quadrant renal transplant nephroureteral catheter exchange. The nephroureteral catheter was placed in 2016 because of the presence of a hematoma causing partial page kidney and hydronephrosis. An antegrade nephrostogram was notable for opacification of the small bowel instead of the renal collecting system.

Effector memory differentiation increases detection of replication-competent HIV-l in resting CD4+ T cells from virally suppressed individuals.

Studies have demonstrated that intensive ART alone is not capable of eradicating HIV-1, as the virus rebounds within a few weeks upon treatment interruption. Viral rebound may be induced from several cellular subsets; however, the majority of proviral DNA has been found in antigen experienced resting CD4+ T cells. To achieve a cure for HIV-1, eradication strategies depend upon both understanding mechanisms that drive HIV-1 persistence as well as sensitive assays to measure the frequency of infected cells after therapeutic interventions.

Crystal structure and Hirshfeld surface analysis of 2-[(2-oxo-2-chromen-4-yl)-oxy]acetic acid dimethyl sulfoxide monosolvate.

The title compound, CHO·(CH)SO, is a new coumarin derivative. The asymmetric unit contains two coumarin mol-ecules ( and ) and two di-methyl-sulfoxide solvent mol-ecules ( and ). The dihedral angle between the pyran and benzene rings in the chromene moiety is 3.

De novo regulation of RD3 synthesis in residual neuroblastoma cells after intensive multi-modal clinical therapy harmonizes disease evolution.

Most high-risk neuroblastomas that initially respond to therapy will ultimately relapse. Currently, no curative treatment is available. Acquired genetic/molecular rearrangement in therapy-resistant cells contributes to tumor relapse.

A Multi-center Study on the Reproducibility of Drug-Response Assays in Mammalian Cell Lines.

Evidence that some high-impact biomedical results cannot be repeated has stimulated interest in practices that generate findable, accessible, interoperable, and reusable (FAIR) data. Multiple papers have identified specific examples of irreproducibility, but practical ways to make data more reproducible have not been widely studied. Here, five research centers in the NIH LINCS Program Consortium investigate the reproducibility of a prototypical perturbational assay: quantifying the responsiveness of cultured cells to anti-cancer drugs.

Emerging concepts in the pathogenesis of the Streptococcus pneumoniae: From nasopharyngeal colonizer to intracellular pathogen.

Streptococcus pneumoniae (the pneumococcus) is a human respiratory tract pathogen and a major cause of morbidity and mortality globally. Although the pneumococcus is a commensal bacterium that colonizes the nasopharynx, it also causes lethal diseases such as meningitis, sepsis, and pneumonia, especially in immunocompromised patients, in the elderly, and in young children. Due to the acquisition of antibiotic resistance and the emergence of nonvaccine serotypes, the pneumococcus has been classified as one of the priority pathogens for which new antibacterials are urgently required by the World Health Organization, 2017.

Genomic Characterization of the Emerging Pathogen Streptococcus pseudopneumoniae.

is a close relative of the major human pathogen It is increasingly associated with lower-respiratory-tract infections (LRTI) and a high prevalence of antimicrobial resistance (AMR). is difficult to identify using traditional typing methods due to similarities with and other members of the mitis group (SMG). Using whole-genome sequencing of LRTI isolates and a comparative genomic approach, we found that a large number of pneumococcal virulence and colonization genes are present in the core genome.

Multiomics Profiling Establishes the Polypharmacology of FDA-Approved CDK4/6 Inhibitors and the Potential for Differential Clinical Activity.

The target profiles of many drugs are established early in their development and are not systematically revisited at the time of FDA approval. Thus, it is often unclear whether therapeutics with the same nominal targets but different chemical structures are functionally equivalent. In this paper we use five different phenotypic and biochemical assays to compare approved inhibitors of cyclin-dependent kinases 4/6-collectively regarded as breakthroughs in the treatment of hormone receptor-positive breast cancer.

The crystal structures of two new coumarin derivatives: 2-(4-{2-[(2-oxo-2-chromen-4-yl)-oxy]acet-yl}piperazin-1-yl)acetamide and -(2,4-di-meth-oxy-benz-yl)-2-[(2-oxo-2-chromen-4-yl)-oxy]acetamide.

The title compounds, 2-(4-{2-[(2-oxo-2-chromen-4-yl)-oxy]acet-yl}piperazin-1-yl)acetamide, CHNO, (I), and -(2,4-di-meth-oxy-benz-yl)-2-[(2-oxo-2-chromen-4-yl)-oxy]acetamide, CHNO, (II), are new coumarin derivatives. In compound (I), the six-membered piperazine adopts a chair conformation. The dihedral angles between the mean planes of the chromene ring and amide plane is 82.

Distant Non-Obvious Mutations Influence the Activity of a Hyperthermophilic Phosphoglucose Isomerase.

The cupin-type phosphoglucose isomerase (PfPGI) from the hyperthermophilic archaeon catalyzes the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate. We investigated PfPGI using protein-engineering bioinformatics tools to select functionally-important residues based on correlated mutation analyses. A pair of amino acids in the periphery of PfPGI was found to be the dominant co-evolving mutation.

2-Hydroxypropyl-β-cyclodextrin is the active component in a triple combination formulation for treatment of Niemann-Pick C1 disease.

Niemann-Pick type C1 (NPC1) disease is a fatal neurovisceral disease for which there are no FDA approved treatments, though cyclodextrin (HPβCD) slows disease progression in preclinical models and in an early phase clinical trial. Our goal was to evaluate the mechanism of action of a previously described combination-therapy, Triple Combination Formulation (TCF) - comprised of the histone deacetylase inhibitor (HDACi) vorinostat/HPβCD/PEG - shown to prolong survival in Npc1 mice. In these studies, TCF's benefit was attributed to enhanced vorinostat pharmacokinetics (PK).

Fine-needle aspiration cytology and cell block in diagnosis and subtyping of hepatoblastoma.

Hepatoblastoma (HB) constitutes less than 1% of all pediatric malignancies and is the most common malignant tumor of liver in children. The fine-needle aspiration cytological (FNAC) diagnosis and sub-typing of this tumor is challenging, which is of critical importance from its treatment point of view. All cases with a clinicoradiological impression of "HB" during the study period of 1 year were subjected to ultrasound-guided (USG) FNAC and cell blocks were prepared in all cases.

Green synthesis of copper oxide nanoparticles and mosquito larvicidal activity against dengue, zika and chikungunya causing vector .

In the present study, high purity copper oxide nanoparticles (NPs) were synthesised using leaf extract. Green syntheses of nano-mosquitocides rely on plant compounds as reducing and stabilising agents. Copper oxide NPs were characterised using X-ray diffraction (XRD) analysis, Fourier transform infrared (FT-IR), Field-emission scanning electron microscopy with energy dispersive spectroscopy, Ultraviolet-visible spectrophotometry and fluorescence spectroscopy.

Patients with Cirrhosis of Liver Operated for Non-transplant Surgery: A Retrospective Analysis.

Patients with cirrhosis of the liver have high mortality after surgery. We investigated the mortality in patients with cirrhosis of the liver who underwent surgery other than liver transplant and applied the Mayo clinic model to predict mortality and compare with the observed mortality. We also studied the association of the observed mortality with the Child-Turcotte-Pugh (CTP) class and the model for end-stage liver disease (MELD) and model for end-stage liver disease-sodium (MELD-Na) scores.

Structural insights on druggable hotspots in CD147: A bull's eye view.

CD147/Basigin/EMMPRIN (Extracellular Matrix Metalloproteinase inducer) is a single pass type1 transmembrane protein playing a central role in developmental process, wound healing, nutrient transport, inflammation, arthritis and also in microbial pathologies. It is also found to be a potent stimulator of MMP (matrix metalloproteinases) and has been considered as a prognostic marker in cancer. Dysregulation of CD147 is reported in several types of cancer.

Commissioning of portal dosimetry using a novel method for flattening filter-free photon beam in a nontrue beam linear accelerator.

Aim: The aim of this study is to commission and validate the portal dosimetry (PD) system using an indirect method for flattening filter free (FFF) photon beam of the upgraded c-series linear accelerator.

Background: Varian Medical System clinacs with amorphous-silicon portal imager panel (aSi-1000) do not have PD for FFF beams. Recently, our c-series linear accelerator was upgraded to deliver 6MV FFF (6MVFFF) photon beam with the highest dose rate of 1400 monitor unit (MU)/min.

A multitargeted probe-based strategy to identify signaling vulnerabilities in cancers.

Most cancer cells are dependent on a network of deregulated signaling pathways for survival and are insensitive, or rapidly evolve resistance, to selective inhibitors aimed at a single target. For these reasons, drugs that target more than one protein (polypharmacology) can be clinically advantageous. The discovery of useful polypharmacology remains serendipitous and is challenging to characterize and validate.