Enhanced Mosquito Larvicidal Efficacy and Dehairing Properties of Bacillus thuringiensis Serovar israelensis Strain VCRC-B649 Isolated from Malabar Coast, India.

In recent years, there has been a global threat from emerging vector-borne diseases (VBD), despite the implementation of several vector control programs. Considering the benefits of bacterial pesticides, the present study aimed to isolate potential mosquitocidal bacteria from the various soil types collected from the Kasaragod (12.5°N, 75.

Molecular identification and GC-MS analysis of a newly isolated novel bacterium (Lysinibacillus sp. VCRC B655) for mosquito control.

Background: Mosquitoes are widespread globally and have contributed to transmitting pathogens to humans and the burden of vector-borne diseases. They are effectively controlled at their larval stages by biocontrol agents. Unravelling natural sources for microbial agents can lead us to novel potential candidates for managing mosquito-borne diseases.

Molecular Characterization of Mosquitocidal Toxin (Surface Layer Protein, SLP) from Bacillus cereus VCRC B540.

A marine Bacillus cereus (VCRC B540) with mosquitocidal effect was recently reported from red snapper fish (Lutjanus sanguineous) gut and surface layer protein (S-layer protein, SLP) was reported to be mosquito larvicidal factor. In this present study, the gene encoding the surface layer protein was amplified from the genomic DNA and functionally characterized. Amplification of SLP-encoding gene revealed 1,518 bp PCR product, and analysis of the sequence revealed the presence of 1482 bp open reading frame with coding capacity for a polypeptide of 493 amino acids.

Synthesis of silver nanoparticles from Azadirachta indica--a most effective method for mosquito control.

Mosquitoes transmit major communicable diseases such as dengue, malaria, filariasis, Japanese encephalitis, chikungunya, and so on. Vector control is important in epidemic disease situations as there is an urgent need to develop new and improved mosquito control methods that are economical and effective yet safe for non-targeted organisms. In the present study, silver nanoparticles (AgNPs) were synthesized from the aqueous leaf extract of neem plant (Azadirachta indica), and their effects on mosquito vectors (Aedes aegypti and Culex quinquefasciatus) were assessed.

Hexamerin a novel protein associated with Bacillus sphaericus resistance in Culex quinquefasciatus.

Bacterial insecticides like, Bacillus sphaericus and Bacillus thuringiensis serovar israelensis, have been used for the control of nuisance and vector mosquitoes for more than two decades. For many years, it was assumed that the use of microbial larvicides based on B. sphaericus would not lead to resistance in mosquitoes.

Identification and characterization of a novel marine Bacillus cereus for mosquito control.

Entomopathogenic bacteria to control mosquitoes are a promising environmentally friendly alternative to synthetic pesticides. In the present study, a novel mosquitocidal bacterium was isolated from marine soil collected from east coastal areas at Pondicherry (India). 16S rRNA gene sequence alignment depicted that this isolate belonged to Bacillus cereus VCRC-B520 (NCBI: KC-119192).

Genotypic diversity of mosquitocidal bacteria (Bacillus sphaericus, B. thuringiensis, and B. cereus) newly isolated from natural sources.

In the present study, to comprehend the genetic diversity of mosquitocidal bacteria, the genotypic analysis of 30 strains of Bacillus species isolated newly from diverse environmental sources has been conducted. Randomly amplified polymorphic DNA polymerase chain reaction was conducted to characterize the genotype diversity between the bacterial strains. The matrix of scores from each bacterial DNA was analyzed, and the correlation between the co-efficients and the similarity matrix data was computed.

A novel cost-effective medium for the production of Bacillus thuringiensis subsp. israelensis for mosquito control.

Bacillus thuringiensis subsp. israelensis (Bti) has been used for mosquito-control programmes the world-wide. Indeed, the large-scale production of Bti for mosquito control is very expensive due to the high cost of its culture.

Coffee husk waste for fermentation production of mosquitocidal bacteria.

Coffee husk waste (CHW) discarded as bio-organic waste, from coffee industries, is rich in carbohydrates. The current study emphasizes the management of solid waste from agro-industrial residues for the production of biopesticides (Bacillus sphaericus, and B. thuringiensis subsp.

Optimization of medium composition for the production of mosquitocidal toxins from Bacillus thuringiensis subsp. israelensis.

Optimization of chicken feather (CF) based culture medium for the production of Bacillus thuringiensis subsp. israelensis (Bti) biomass in combination with the agro industrial by-product (coconut cake, CC) and manganese chloride (MnCl2) has been evaluated. The biomass yield of Bti spore/crystal toxin was highest (12.

A medium for the production of biopesticides (Bacillus sphaericus and Bacillus thuringiensis subsp. israelensis) in mosquito control.

Chicken feathers, discarded as bioorganic wastes from poultry processing industries, are rich in keratin (structural protein). Deoiled rice bran (DRB), in contrast, is an agrowaste product consisting of protein, fiber, and carbohydrates. This study highlights a new medium for producing biopesticides from these bioorganic wastes, by culturing Bacillus sphaericus (Bs) and B.

Novel fermentation media for production of Bacillus thuringiensis subsp. israelensis.

The production of Bacillus thuringiensis subsp. israelensis (deBarjac) (Bti) as a biopesticide is not cost-effective using existing fermentation technology. In this study, we explored the use of several less expensive alternative culture media (potato, common sugar, and Bengal gram) for the growth and production of Bti.