Surface plasmon resonance immunosensor for label-free detection of BIRC5 biomarker in spontaneously occurring canine mammary tumours.

We report detection of Baculoviral inhibitor of apoptosis repeat containing-5 (BIRC5) protein biomarker in dog serum by label-free surface plasmon resonance (SPR) immunosensor. Initially, overexpression of BIRC5 in canine mammary tumour (CMT) tissues was confirmed by real-time PCR. Recombinant BIRC5 was produced and protein specific antibodies developed in guinea pig specifically reacted with native protein in immunohistochemistry and immunocytochemistry.

Multiplexed Autoantibody Signature for Serological Detection of Canine Mammary Tumours.

Spontaneously occurring canine mammary tumours (CMTs) are the most common neoplasms of female unspayed dogs and are of potential importance as models for human breast cancer as well. Mortality rates are thrice higher in dogs as compared to humans with breast cancer, which can partly be attributed to lack of diagnostic techniques for their early detection. Human breast cancer studies reveal role of autoantibodies in early cancer diagnosis and also the usefulness of autoantibody panels in increasing the sensitivity, as well as, specificity of diagnostic assays.

Trypanosoma evansi infection in a German shepherd dog - Apparent successful treatment using serial low dose of diminazene aceturate.

The present study reports a new case of Trypanosoma evansi infection in a dog possibly due to oral transmission from a wild antelope, Nilgai. A four year old male German shepherd dog presented to the Referral Veterinary Polyclinic with history of inappetance, persistent fever, ocular discharge, standing for prolong periods and head pressing. Physical examination revealed corneal opacity, absence of menace reflex and partial blindness.