A new alkaline pectin lyase with novel thermal and pH stability from Bacilus velezensis.

Pectin lyases are important in various industries, including tobacco leaves processing. In this paper, a novel pectin lyase Pel04 from Bacillus velezensis was characterized. Pel04 molecular weight (Mw) and isoelectric point (pI) of the protein sequence after removing the signal peptide are 43.

Predictive factors for pleural drainage volume after uniportal video-assisted thoracic surgery lobectomy for non-small cell lung cancer: a single-institution retrospective study.

Objective: To identify the predictive factors associated with pleural drainage volume (PDV) after uniportal video-assisted thoracic surgery (VATS) lobectomy for non-small cell lung cancer (NSCLC).

Methods: A total of 440 consecutive NSCLC patients who underwent uniportal VATS lobectomy were enrolled in this study between November 2016 and July 2019. Thirty-four parameters, including patients' clinicopathological characteristics and other potential predictors were collected.

Spectroscopic Study on the Interaction of Human Cytoglobin with Copper(Ⅱ) Ion.

Cytoglobin (Cygb), a recently discovered member of the vertebrate globin family, exhibits a traditional globin fold with a three-over-three α-helical sandwich. The interaction between copper(Ⅱ) ion (Cu2+) and Cygb has been investigated by UV-Vis, fluorescence, synchronous fluorescence and circular dichroism (CD) spectra. Results showed that the absorption intensity of Cygb at 280 nm increased and the intrinsic fluorescence of Cygb was quenched when Cu2+ was added.