DNA-hosted Hoechst dyes: application for label-free fluorescent monitoring of endonuclease activity and inhibition.

A simple and facile approach was developed for monitoring EcoRI endonuclease activity and inhibition, in which a hairpin-like DNA containing restriction cutting site for EcoRI endonuclease acts as the sensing element and Hoechst dyes as the signal indicator in a label-free format.

A cost-effective Z-folding controlled liquid handling microfluidic paper analysis device for pathogen detection via ATP quantification.

A cost-effective microfluidic paper analysis device (μPAD) was developed with a special Z-folding design for controlling the fluidic flowing and substrate transportation. This presented μPAD can be easily fabricated through wax printing by using a solid ink printer which deposits wax onto the surface of a chromatographic paper, and then baked on a hotplate by penetrating the molten wax into the paper to create a hydrophobic barrier. After μPAD fabrication, liquid control and substrate transportation can be easily carried out by twice folding the μPAD following Z shape.

A ratiometric fluorescent probe for sensitive, selective and reversible detection of copper (II) based on riboflavin-stabilized gold nanoclusters.

Most of the copper (II) fluorescent probes are based on the measurement of fluorescence at a single wavelength, which may be influenced by variations in the sample environment. To the end, the ratiometric fluorescent measurement, which involves the simultaneous measurement of two fluorescence signals at different wavelengths followed by calculation of their intensity ratio, can effectively eliminate the adverse effects on fluorescence signals and give greater precision to the data analysis relative to single-channel detection. In this work, we prepared novel luminescent gold nanoclusters (AuNCs) utilizing vitamin B2 (riboflavin) as stabilizer by a simple, rapid and one-pot green (low-toxicity materials use) procedure.

A label-free fluorescent molecular beacon based on DNA-templated silver nanoclusters for detection of adenosine and adenosine deaminase.

A simple and reliable fluorescent molecular beacon is developed utilizing DNA-templated silver nanoclusters as a signal indicator and adenosine triphosphate (ATP) and adenosine deaminase as mechanical activators.