Parental Postoperative Pain Management Perceptions, Attitudes, and Practices in Pediatric Limb Fractures.

Background: Post-surgical pain in children is common, severe, and inadequately controlled. An effective model should involve the participation of parents.

Aims: To investigate parental perceptions, attitudes, and practices in postoperative pain management in children with limb fractures and analyze the factors affecting parental practices.

Modeling Notch-Induced Tumor Cell Survival in the Ovary Identifies Cellular and Transcriptional Response to Nuclear NICD Accumulation.

Notch is a conserved developmental signaling pathway that is dysregulated in many cancer types, most often through constitutive activation. Tumor cells with nuclear accumulation of the active Notch receptor, NICD, generally exhibit enhanced survival while patients experience poorer outcomes. To understand the impact of NICD accumulation during tumorigenesis, we developed a tumor model using the ovarian follicular epithelium.

Studies on the role of IS1216E in the formation and dissemination of poxtA-carrying plasmids in an Enterococcus faecium clade A1 isolate.

Objectives: To analyse the role of IS1216E in the dissemination of the phenicol-oxazolidinone-tetracycline resistance gene poxtA in an Enterococcus faecium clade A1 isolate.

Methods: MICs were determined by broth microdilution. The poxtA-positive isolate was typed by MLST.

Analysis of two pheromone-responsive conjugative multiresistance plasmids carrying the novel mobile locus from .

The acquired gene, which encodes a ribosomal protection protein of the ABC-F family, can confer cross-resistance to linezolid and florfenicol, posing a serious therapeutic challenge to both human and veterinary medicine. The objective of this study was to investigate the two () plasmids for their fine structure, their transferability and the presence of mobile antimicrobial resistance loci. To elucidate their fine structure, the two plasmids were completely sequenced and the sequences analysed.

A prophage and two ICESa2603-family integrative and conjugative elements (ICEs) carrying optrA in Streptococcus suis.

Objectives: To investigate the presence and transfer of the oxazolidinone/phenicol resistance gene optrA and identify the genetic elements involved in the horizontal transfer of the optrA gene in Streptococcus suis.

Methods: A total of 237 S. suis isolates were screened for the presence of the optrA gene by PCR.

Tn Is a Novel Enterococcal -Carrying Multiresistance Transposon of the Tn Family.

The novel 12,932-bp nonconjugative multiresistance transposon Tn was identified in the chromosomal DNA of a porcine strain. Tn belongs to the Tn family of transposons. It shares the same arrangement of the transposase genes , and with Tn However, in addition to the Tn-associated resistance genes and (A), Tn harbored the resistance genes and Circular forms of Tn were detected and suggest the functional activity of this transposon.

Analysis of a poxtA- and optrA-co-carrying conjugative multiresistance plasmid from Enterococcus faecalis.

Objectives: To investigate the presence and transferability of the poxtA gene and identify the genetic context of poxtA in two enterococcal plasmids from swine.

Methods: MICs were determined by broth microdilution. A total of 114 porcine enterococci with florfenicol MICs of ≥16 mg/L were screened for the presence of the poxtA gene by PCR.

Biomarkers enhance the long-term predictive ability of the KAMIR risk score in Chinese patients with ST-elevation myocardial infarction.

Background: The Global Registry of Acute Coronary Events (GRACE) score is recommended by current ST-elevation myocardial infarction (STEMI) guidelines. But it has inherent defects. The present study aimed to investigate the more compatible risk stratification for Chinese patients with STEMI and to determine whether the addition of biomarkers to the Korea Acute Myocardial Infarction Registry (KAMIR) score could enhance its predictive value for long-term outcomes.

Doppler myocardial performance index combined with plasma B-type natriuretic peptide levels as a marker of cardiac function in patients with decompensated cirrhosis.

Background: In chronic liver diseases, cirrhosis ranks as the 14th highest death cause worldwide, developing into decompensated cirrhosis. A potential and feasible technique in assessing cardiac function is urgent. This study explores if the Doppler myocardial performance (Tei) index combined with the plasma B-type natriuretic peptide (BNP) levels can assess cardiac function in patients with decompensated cirrhosis.

In vivo effects of the NLRP1/NLRP3 inflammasome pathway on latent respiratory virus infection.

The present study aimed to investigate the effects of nucleotide-binding domain leucine-rich repeat protein (NLRP)1/NLRP3 inflammasome pathways on latent viral infection of the respiratory tract. A total of 55 BALB/c mice were assigned to the control, bleomycin (BLM)‑treated, murine cytomegalovirus (MCMV), MCMV+BLM and MCMV+BLM+CD4+ T‑cell groups. The viral loads were detected in the salivary glands, kidney, liver and lung tissues via polymerase chain reaction (PCR).

Melatonin inhibits the Migration of Colon Cancer RKO cells by Down-regulating Myosin Light Chain Kinase Expression through Cross-talk with p38 MAPK.

Background: Melatonin, which is mainly produced by the pineal gland, has a good inhibitory effect on cell growth of multiple cancer types. However, the underlying molecular mechanisms of anti-tumor activity for colon cancer have not been fully elucidated. In this study, we investigated the effects of melatonin on migration in human colon cancer RKO cells and the potential molecular mechanisms.

[Modified method for purifying Cryptosporidium oocysts].

Sheather's sucrose solution diluted with distilled water as an alternative to PBS was used to purify oocysts of three different Cryptosporidium spp. The recovery rate of purified Cryptosporidium andersoni, C. baileyi and C.

μ-Bis(5,7-dimethyl-1,8-naphthyridin-2-yl)diazene]bis-[difluoridoboron(III).

In the title compound, C(20)H(18)B(2)F(4)N(6), the bis-(5,7-dimethyl-1,8-naphthyridin-2-yl)diazene molecule is bis-ected by a symmetry centre midway between the central N atoms of the diazene group. Each of the symmetry-related halves of the molecule binds to a B atom through an N,N'-bite. Two terminal F ions complete the distorted BN(2)F(2) tetra-hedral geometry around each B atom.

Correlation between expression and differentiation of endocan in colorectal cancer.

Aim: To investigate the expression frequency of endocan in colorectal cancer and analyze the relationship between endocan expression and clinical parameters and to study the role of endocan in colorectal carcinogenesis.

Methods: Expression of endocan in 72 tumor tissue samples of colorectal cancer as well as in 27 normal mucous membrane tissue samples was analyzed using in situ hybridization, immunohistochemistry on tissue microarray, Western blot and reverse-transcript polymerase chain reaction (RT-PCR).

Results: The expression of endocan was higher in normal colon and rectum tissue samples than in cancerous tissue samples (mRNA = 92.

2,2'-Dimethyl-7,7'-(methyl-enediimino)di-1,8-naphthyridin-1-ium bis-(perchlorate).

In the title salt, C(19)H(20)N(6) (2+)·2ClO(4) (-), the two planar 1,8-naphthyridine systems are linked by a methyl-enediamine group with a dihedral angle of 60.6 (1)° between the two systems. The crystal structure involves extensive N-H⋯O and C-H⋯O hydrogen bonding.

{μ-N-[(Diphenyl-phosphino)meth-yl]pyridin-2-amine-κN:P}bis-{[2-(2,2'-bipyridin-6-yl)phenyl-κN,N',C]platinum(II)} bis-(perchlorate).

The title compound, [Pt(2)(C(16)H(11)N(2))(2)(C(18)H(17)N(2)P)](ClO(4))(2), contains two Pt(II) atoms, bridged by an N-[(diphenyl-phosphino)meth-yl]pyridin-2-amine (dppmp) ligand. One Pt atom is coordinated by one P atom from the dppmp ligand, and one C atom and two N atoms from a 6-phenyl-2,2'-bipyridine (pbpy) ligand in a square-planar geometry. The other Pt atom is coordinated by one N atom from the dppmp ligand, and one C atom and two N atoms from another pbpy ligand in a square-planar geometry.

Hexa-μ(2)-acetato-1:2κO:O';1:2κO:O;2:3κO:O';2:3κO:O-bis-(2-amino-7-chloro-5-methyl-1,8-naphthyridine)-1κN,3κN-trizinc(II).

The title complex, [Zn(3)(C(2)H(3)O(2))(6)(C(9)H(8)ClN(3))(2)], contains three Zn(II) atoms bridged by six acetate ligands. The central Zn(II) ion, located on an inversion centre, is surrounded by six O atoms from acetate ligands in a distorted octa-hedral geometry [Zn-O = 1.9588 (12)-2.

(μ-Diphenyl-phosphanido-κP:P')bis-[2,2'-(pyridine-2,6-diyl)diphenyl-κC,N,C)gold(III)] perchlorate acetonitrile solvate.

The title complex, [Au(2)(C(17)H(11)N)(2)(C(12)H(10)P)]ClO(4)·C(2)H(3)N, contains two Au(III) atoms bridged by a diphenyl-phosphanide ligand. Each Au atom is in a square-planar environment coordinated by diphenyl-phosphanide and 2,6-diphenyl-pyridine ligands. There are weak π-π stacking inter-actions between neighbouring mol-ecules (the inter-planar separations between two neighbouring dpp units are 3.

Antimicrobial susceptibility and molecular detection of chloramphenicol and florfenicol resistance among Escherichia coli isolates from diseased chickens.

Seventy Escherichia coli isolates recovered from diseased chickens diagnosed with colibacillosis in Henan Province, China, between 2004 and 2005 were characterized for antimicrobial susceptibility profiles via a broth doubling dilution method. Overall, the isolates displayed resistance to trimethoprim-sulfamethoxazole (100%), oxytetracycline (100%), ampicillin (83%), enrofloxacin (83%), and ciprofloxacin (81%), respectively. Among the phenicols, resistance was approximately 79% and 29% for chloramphenicol and florfenicol, respectively.

Transcriptional levels of growth factors in skeletal muscle of maintenance hemodialysis patients.

This study was performed to assess the mRNA levels in skeletal muscle of maintenance hemodialysis (MHD) patients. Fifty-one sedentary MHD patients and 21 sedentary normal adults of similar age, gender distribution, and racial/ethnic mix as the patients were examined. The subjects had needle muscle biopsy to measure mRNA levels.