Forensic Validation and Application Evaluation of IDentifier DNA Typing Kit (Yan-Huang34).

Objectives: To investigate the technical performance of IDentifier DNA typing kit (YanHuang34) and evaluate its forensic application value.

Methods: Following the (GB/T 37226-2018), IDentifier DNA typing kit (YanHuang34) was verified in 11 aspects of species specificity, veracity, sensibility, adaptability, inhibitor tolerance, consistency, balance, reaction condition verification, mixed samples, stability and inter batch consistency. The system efficiency of IDentifier DNA typing kit (YanHuang34) was compared with the PowerPlex Fusion 6C System, VersaPlex 27PY System and VeriFiler Plus PCR Amplification Kit.

Molecular investigation of infection sources and transmission chains of brucellosis in Zhejiang, China.

In the present study, a total of 7793 samples from 5 different types of hosts were collected and tested, with a seroprevalence of 2.4% (184/7793). Although the seroprevalence of human and animal brucellosis is relatively low, numbers of human brucellosis cases reported have increased continuously from 2004 to 2018.