[Sequencing and analysis of the complete genome of encephalomyocarditis virus strain GXLC isolated from swine].

The complete genome of encephalomyocarditis virus (EMCV)strain GXLC isolated from swine was sequenced and analyzed. Five overlapped gene fragments covering the entire open reading frame (ORF) were amplified by RT-PCR, and the 3'-untranslated region (UTR) and 5'-UTR were amplified by the 3'-rapid amplification of cDNA ends (RACE) and 5'-RACE method, respectively. The genome sequences of strain GXLC were obtained by assembling the sequences of RT-PCR-generated cDNA fragments.

[The diagnostic value of flexible bronchoscopy in tracheobronchopathia osteochondroplastica].

Objective: To enhance the knowledge of tracheobronchopathia osteochondroplastica (TO), and to describe the value of flexible bronchoscopic diagnosis and treatment for the disease.

Methods: The clinical data, bronchoscopic findings, histological results and the methods and effect of bronchoscopic treatment in 10 patients with TO admitted to Xiangya Hospital between June 2006 and July 2007 were retrospectively analyzed.

Results: There were 8 males and 2 females (mean age 46 +/- 16, range 33-76 years).

[Medical thoracoscopy in the diagnosis of complicate pleural effusion].

Objective: To evaluate the role of medical thoracoscopy in the diagnosis of the pleural effusion of unknown etiology.

Methods: The results of 36 patients with the pleural disease of unknown etiology diagnosed by medical thoracoscopy were retrospectively analyzed, including the pathologic results and the complications.

Results: Among the 36 patients, 35 were determined with positive rate of 97.