The Prospect of Hydrolytic Enzymes from Species in the Biological Control of Pests and Diseases in Forest and Fruit Tree Production.

Plant diseases and insect pest damage cause tremendous losses in forestry and fruit tree production. Even though chemical pesticides have been effective in the control of plant diseases and insect pests for several decades, they are increasingly becoming undesirable due to their toxic residues that affect human life, animals, and the environment, as well as the growing challenge of pesticide resistance. In this study, we review the potential of hydrolytic enzymes from species such as chitinases, β-1,3-glucanases, proteases, lipases, amylases, and cellulases in the biological control of phytopathogens and insect pests, which could be a more sustainable alternative to chemical pesticides.

Entomopathogenic Potential of CE 100 for the Biological Control of Termite Damage in Wooden Architectural Buildings of Korean Cultural Heritage.

Biocontrol strategies are gaining tremendous attention in insect pest management, such as controlling termite damage, due to the growing awareness of the irreparable harm caused by the continuous use of synthetic pesticides. This study examines the proteolytic and chitinolytic activities of CE 100 and its termiticidal effect through cuticle degradation. The proteolytic and chitinolytic activities of CE 100 systematically increased with cell growth to the respective peaks of 68.

Biological Control of Leaf Blight Disease Caused by and Growth Promotion of Carruth Container Seedlings Using CE 100.

Leaf blight disease caused by lead to deleterious losses in the quality of forest container seedlings. The use of plant growth-promoting bacteria provides a promising strategy to simultaneously control diseases and enhance forest seedling production. This study investigated the biocontrol of leaf blight disease and growth promotion potential of CE 100 in Carruth seedlings.

Antifungal Activity of CE 100 against Anthracnose Disease () and Growth Promotion of Walnut ( L.) Trees.

Walnut anthracnose caused by is a deleterious disease that severely affects the production of walnut ( L.). The aim of this study was to assess the antifungal and growth promotion activities of CE 100 as an alternative to chemical use in walnut production.

The Role of HS124 on the Control of Fall Webworm ( Drury) and Growth Promotion of Canadian Poplar ( Moench) at Saemangeum Reclaimed Land in Korea.

Moench forests established in Saemangeum-reclaimed land have been invaded by Drury, causing defoliation and stunted growth. This study investigated the biocontrol potential of cuticle degrading chitinase and protease secreted by HS124 against larvae. In addition, HS124 was examined for indole-3-acetic acid phytohormone production for plant growth promotion.

CE 100 Inhibits Root Rot Diseases ( spp.) and Promotes Growth of Japanese Cypress ( Endlicher) Seedlings.

Root rot diseases, caused by phytopathogenic oomycetes, spp. cause devastating losses involving forest seedlings, such as Japanese cypress ( Endlicher) in Korea. Plant growth-promoting rhizobacteria (PGPR) are a promising strategy to control root rot diseases and promote growth in seedlings.

Heme Derived from : A Potential Iron Additive for Swine and an Electron Carrier Additive for Lactic Acid Bacterial Culture.

To investigate the potential applications of bacterial heme, aminolevulinic acid synthase (HemA) was expressed in a HA strain that had been adaptively evolved against oxidative stress. The red pigment from the constructed strain was extracted and it exhibited the typical heme absorbance at 408 nm from the spectrum. To investigate the potential of this strain as an iron additive for swine, a prototype feed additive was manufactured in pilot scale by culturing the strain in a 5 ton fermenter followed by spray-drying the biomass with flour as an excipient (biomass: flour = 1:10 (w/w)).

Growth retardation of Escherichia coli by artificial increase of intracellular ATP.

Overexpression of phosphoenolpyruvate carboxykinase (PCK) was reported to cause the harboring of higher intracellular ATP concentration in Escherichia coli, accompanied with a slower growth rate. For systematic determination of the relationship between the artificial increase of ATP and growth retardation, PCKWT enzyme was directly evolved in vitro and further overexpressed. The evolved PCK67 showed a 60% greater catalytic efficiency than that of PCKWT.

Analysis of Heme Biosynthetic Pathways in a Recombinant Escherichia coli.

Bacterial heme was produced from a genetic-engineered Escherichia coli via the porphyrin pathway and it was useful as an iron resource for animal feed. The amount of the E. colisynthesized heme, however, was only few milligrams in a culture broth and it was not enough for industrial applications.