Intervening or interfering? The influence of injunctive and descriptive norms on intervention behaviours in alcohol consumption contexts.

In situations when people have been drinking, they often find it difficult to tell their friends to stop drinking, or not to drive home. Most people want to avoid being seen as a busybody, which may inhibit advice giving. In the current study, we investigated how positive and negative descriptive and injunctive norms (in alcohol consumption contexts) affect people's motivation to engage in intervening (e.

Understanding the antecedents of Korean high school students' drinking refusal self-efficacy: parental influence, peer influence, and behavior.

The current study examined the factors that influence Korean adolescents' drinking refusal self-efficacy, which is known to be associated with alcohol use and drinking intentions. Specifically, this study considered parental monitoring, parent-child communication satisfaction, peer influence, and prior alcohol use as possible antecedents of Korean high school students' drinking refusal self-efficacy. High school students (n = 538) in South Korea responded to the current study.

Normative influences and alcohol consumption: the role of drinking refusal self-efficacy.

This article extends the theory of normative social behavior by conceptualizing drinking refusal self-efficacy as an important moderator in the relationship between descriptive norms and drinking intentions and behavior. A study was conducted among Korean high school students (N = 538) to assess their normative perceptions, drinking refusal self-efficacy, and drinking intentions. We found a significant association between self-efficacy and drinking intentions and behavior, as well as an interaction effect between self-efficacy and descriptive norms on drinking intentions and behavior.

Mechanism of action and specificity of antimicrobial peptides designed based on buforin IIb.

Buforin IIb-a synthetic analog of buforin II that contains a proline hinge between the two α-helices and a model α-helical sequence at the C-terminus (3× RLLR)-is a potent cell-penetrating antimicrobial peptide. To develop novel antimicrobial peptides with enhanced activities and specificity/therapeutic index, we designed several analogs (Buf III analogs) by substitutions of amino acids in the proline hinge region and two α-helices of buforin IIb, and examined their antimicrobial activity and mechanism of action. The substitution of hydrophobic residues ([F(6)] and [V(8)]) in the proline hinge region with other hydrophobic residues ([W(6)] and [I(8)]) did not affect antimicrobial activity, while the substitution of the first four amino acids RAGL with a model α-helical sequence increased the antimicrobial activity up to 2-fold.

Self-monitoring as a moderator between descriptive norms and drinking: findings among Korean and American university students.

Guided by the theory of normative social behavior (TNSB), this research examined whether a self-monitoring individual difference variable moderates the link between descriptive norms and drinking as well as drinking intentions such that the relations become stronger as self-monitoring becomes stronger. Contrary to our prediction, Study 1 showed that low self-monitoring Korean undergraduates were more likely to be guided by normative information when drinking and intending to drink when compared to those with high self-monitors. Study 2 was conducted using an American university sample, and results of the second study were identical to those of the first study.

Engineering butanol-tolerance in escherichia coli with artificial transcription factor libraries.

Escherichia coli has been explored as a host for butanol production because of its many advantages such as a fast growth and easy genetic manipulation. Butanol toxicity, however, is a major concern in the biobutanol production with E. coli.

Direct expression of antimicrobial peptides in an intact form by a translationally coupled two-cistron expression system.

We describe a novel prokaryotic expression system for the production of cationic antimicrobial peptides (AMPs). The method relies on a translationally coupled two-cistron system, in which the termination codon for the first cistron (which encodes the anionic polypeptide mIFc2, a derivative of human gamma interferon) overlaps with the initiation codon for the second cistron (which encodes a cationic AMP) in the sequence of 5'-TAATG-3'. By forming an insoluble complex with the AMP upon translation, the mIFc2 protein efficiently neutralized the toxicity of the coexpressed cationic AMP and minimized the sensitivity of AMP to proteolytic degradation in a host.

Mechanism of anticancer activity of buforin IIb, a histone H2A-derived peptide.

Buforin IIb is a novel cell-penetrating anticancer peptide derived from histone H2A. Here we analyzed the anticancer activity and cancer cell-killing mechanism of buforin IIb. Buforin IIb displayed selective cytotoxicity against 62 cancer cell lines by specifically targeting cancer cells through interaction with cell surface gangliosides.

Structure-activity relations of parasin I, a histone H2A-derived antimicrobial peptide.

The structure-activity relations and mechanism of action of parasin I, a 19-amino acid histone H2A-derived antimicrobial peptide, were investigated. Parasin I formed an amphipathic alpha-helical structure (residues 9-17) flanked by two random coil regions (residues 1-8 and 18-19) in helix-promoting environments. Deletion of the lysine residue at the N-terminal [Pa(2-19)] resulted in loss of antimicrobial activity, but did not affect the alpha-helical content of the peptide.

High-level expression of an antimicrobial peptide histonin as a natural form by multimerization and furin-mediated cleavage.

Direct expression of an antimicrobial peptide (AMP) in Escherichia coli causes several problems such as the toxicity of AMP to the host cell, its susceptibility to proteolytic degradation, and decreased antimicrobial activity due to the additional residue(s) introduced after cleavage of AMPs from fusion partners. To overcome these problems and produce a large quantity of a potent AMP histonin (RAGLQFPVGKLLKKLLKRLKR) in E. coli, an efficient expression system was developed, in which the toxicity of histonin was neutralized by a fusion partner F4 (a truncated fragment of PurF protein) and the productivity was increased by a multimeric expression of a histonin gene.