Inhibition of follicle stimulating hormone binding to granulosa cells in vitro by human follicular fluid.

Evidence for the existence of a low molecular weight (less than 10,000 daltons) factor in human follicular fluid that inhibits binding in vitro of FSH to ovarian granulosa cells is presented.

Study of testicular feedback in male rats using artificial cryptorchidism as a model.

Plasma LH, FSH and testosterone were measured in testosterone-treated and untreated cryptorchid and castrated male rats. Exogenous testosterone prevented the increase in basal LH but not FSH levels seen in the untreated cryptorchids. Increases in plasma LH and FSH in response to LH-RH were greater in the cryptorchid as compared to the control group and this could not be reversed by exogenous testosterone, suggesting that spermatogenesis-related feedback factors regulate LH as well as FSH at the pituitary level in the intact rat.

Intra-ovarian formation of steroid sulphates: measurement and influence of gonadotrophins.

The steroid sulphokinase activity in 105 000 g supernatant fractions from rat and bovine ovarian tissue has been determined by measuring the radioactivity incorporated into conjugated steroids after incubation with 14C-labelled androgens and oestrogens. The enzyme from bovine ovaries was partially characterized, changes in its activity with pH were measured and Km values of 30, 10 and 4 mumol/1 were obtained for testosterone, oestradiol and oestrone respectively. The rate of formation of sulphates from oestrogens by preparations of bovine luteal tissue was 10--20 times higher than the rate of formation by follicular tissue (P less than 0.

[Inhibin-like activity in human ovarian follicular fluid (author's transl)].

A steroid-free proteinaceous substance with inhibin-like activity has been extracted from human ovarian follicular fluid by alcohol precipitation. This substance was capable of inhibiting the ovarian weight increase of hCG-treated immature female rats and also of suppressing the post-castration rise of serum FSH levels in immature male rats. Follicular fluid from small follicls obtained from regularly cycling women was--on the basis of protein- found to be more active than cystic follicular fluid obtained from a premenopausal woman.

[The action of 1-aminoadamantane. Comparative studies with isolated nerve endings and thrombocytes on the release of serotonin and dopamine].

Nerve endings isolated from rat brain accumulate exogenous serotonin and dopamine. Both biogenic amines are released from nerve endings as well by means of electrical stimulation with rectangular pulses of alternating polarity as by incubation with 5 X 10(-4) to 5 X 10(-5) M solutions of the adamantane derivatives 1-aminoadamantane hydrochloride (D 1) and 1-amino-3,5-dimethyladamantane hydrochloride (memantine, D 145). The electrically stimulated liberation is small but is significantly increased after simultaneous incubation of the nerve endings with subthreshold concentrations of 1-aminoadamantanes (5X10(-6) to 5X10(-5) M).

[Ultrasonics of ovarian changes under gonadotrophine stimulation (author's transl)].

It is known that there are difficulties in timing of ovulation induction under gonadotrophines. The reason is that investigation parameters used so far only permit indirect quantitative statements. Ultrasonics permit the direct presentation of ovaries, cystic structure-follicles, registration of their number and size as well as control of the further development.

Investigations into the preservation of human ovaries by means of a cryoprotectivum.

Human ovaries in the phase of follicular ripening were treated by means of a cryoprotectivum immediately after their removal. They were kept at --18degrees C for up to 8 weeks. After having gradually been thawed the ovaries were in vitro connected to a perfusion system.

[Gaschromatographic method for routine determination of pregnanediol and pregnanetriol in urine (author's transl)].

A rapid, highly sensitive and simple method for the routine measurement of urinary pregnanediol and pregnanetriol involving enzymatic hydrolysis, extraction with toluene and gas liquid chromatography is described.

Effect of 13-Nle-motilin on small intestinal transit time in healthy subjects.

In 6 female subjects without gastrointestinal diseases, 13-norleucine motilin (13-Nle-M) synthetic and biological analogue of the duodenal polypeptide, motilin, caused acceleration of intestinal transit time. Intravenous infusion of 0.4 mug/kg-h of 13-Nle-M reduced mean transit time by 50 percent.

[Multilocular tumors of small intestine (author's transl)].

Tumors of small intestine can be recognized in time and evaluated histologically by means of roentgenographic and endoscopical-bioptic methods, respectively. Among radiologic techniques, the double contrast examination of the small intestine has especially high diagnostic value. On the other hand, selective angiography reveals bleeding of accompanying small intestinal tumors.

Testosterone undecanoate: a new orally active androgen.

Oral testosterone undecanoate (TU) in arachis oil has been evaluated with a view to its possible use as a means of androgen replacement therapy. A single 100 mg dose was found to elevate plasma androgen levels and urinary 17-ketosteroid excretion in 6 normal men. Ninety mg/day and 60 mg/day doses taken by a hypogonadal man resulted in sustained levels of androgen which appeared physiological when measured by radioimmunoassay without chromatography.

[Blood clotting and thrombocyte function in reversible hemorrhagic shock].

In experiments on 12 mongrel dogs, serving as models of reversible hemorrhagic shock, the influence on blood clotting and thrombocyte function was studied. Initial hypercoagulability, observed already after introduction of anesthesia, and multiplying of pathological aggregation stages dissociated from decreased number of thrombocytes suggest a considerable influence of the proceeding of reversible hemorrhagic shock on the clotting system. It is concluded that the initial hypercoagulability is not causally related to the changes of platelet function, which represent a starting mechanism for the arising of consumptive reaction in protracted and irreversible shock.

Optical wave measurement technique and experimental comparison with conventional wave height probes.

An optical system developed to measure wave slope is described. When the phase speed of a wave train propagating in a single direction is known, the measured slope can be integrated to give wave height directly. Results obtained under these conditions are compared with those from a capacitive wave height probe and demonstrate the accuracy of the system.