Comparison of blood and brain toluene concentrations and circulating triglyceride levels resulting from acute and repeated exposures in rats.

Male Sprague-Dawley rats were exposed by inhalation to 2500 ppm toluene three hours per day, five days per week for three weeks. Blood and brain toluene concentration and circulating triglyceride levels were measured after one day, one week, two weeks and three weeks exposure. A consistent reduction in mean body weight gain was found in the toluene-exposed rats compared to controls as early as three days following initiation of exposure.

Simplified detection of hydrochlorothiazide in urine by thin-layer chromatography.

A new thin-layer chromatographic procedure for the detection of the antihypertensive/diuretic drug hydrochlorothiazide in human urine is described. The sensitivity of the method, 5 micrograms/mL, makes it useful to both the clinician interested in determining patient compliance and the toxicologist who must identify or confirm hydrochlorothiazide ingestion. The rapidity of analysis typically allows the screening of 25 urine samples within one hour.

Gas-chromatographic determination of amantadine in human urine.

We describe a gas--liquid-chromatographic method for determining the concentration of amantadine hydrochloride in urine with beta-phenylethylamine as internal standard. The urine sample is made alkaline and extracted with 0.5 mL of chloroform.