Incubation of exogenous fatty acids with lymphocytes. Changes in fatty acid composition and effects on the rotational relaxation time of 1,6-diphenyl-1,3,5-hexatriene.

Lymphocytes were incubated with various long chain fatty acids bound to albumin. Incubation for 20 h with unsaturated fatty acids resulted in uptake into the neutral lipids and phospholipids. The addition of concanavalin A enhanced the uptake.

The isolation and analysis of the luminal plasma membrane of calf urinary bladder epithelium.

The luminal plasma membrane of calf urinary bladder epithelium (urothelium) has been isolated by a method designed to preserve enzymic activity as well as structural integrity. The yield was about 80 micrograms per calf bladder. Low levels of 5' nucleotidase, Mg2+-ATPase and (Na+ + K+)-ATPase activities were found in the luminal membrane fraction.

Application of flow microcalorimetry to analytical problems-I. Determination of organophosphorus pesticides by inhibition of cholinesterase.

Flow microcalorimetry has been applied to the determination of organophosphorus pesticides by inhibition of cholinesterase. One direct inhibitor (TEPP) and one latent inhibitor (parathion) were investigated. The former is determinable at concentrations of about 10(-6)M and the latter at about 10(-4)M.