Optimization and Validation of an 8-Color Single-Tube Assay for the Sensitive Detection of Minimal Residual Disease in B-Cell Chronic Lymphocytic Leukemia Detected via Flow Cytometry.

Objective: The European Research Initiative on chronic lymphocytic leukemia (ERIC) has designed a single-tube, 8-color chronic lymphocytic leukemia CLL-MRD flow cytometric assay to standardize testing in patients with B-CLL. This study aims to optimize and validate the 8-color CLL-MRD assay, with the desired outcome of it being implemented in St. James's Hospital Dublin and potentially other hospitals worldwide as the most accurate flow-based B-CLL-MRD detection currently available.

An immunophenotypic and molecular diagnosis of composite hairy cell leukaemia and chronic lymphocytic leukaemia.

Hairy cell leukaemia (HCL) and chronic lymphocytic leukaemia (CLL) are distinct clinicopathological B cell chronic lymphoproliferative disorders (B-CLPD). Both diseases have characteristic immunophenotypic and molecular features. The co-existence of two B-CLPD is perhaps more common than previously thought but a composite HCL and CLL has been rarely documented.