Complexome profiling is an experimental approach to identify interactions by integrating native separation of protein complexes and quantitative mass spectrometry. In a typical complexome profile, thousands of proteins are detected across typically ≤100 fractions. This relatively low resolution leads to similar abundance profiles between proteins that are not necessarily interaction partners.
View Article and Find Full Text PDFObjective: To explore and describe the everyday practices (Work-As-Done) that hinder and facilitate patient care transitions from the intensive care unit (ICU) to the ward.
Research Method/design: Multiple qualitative case studies in the ICU and various specialized wards of three Dutch hospitals. Adult patients planned to be transferred were purposively sampled on a variety of characteristics along with their relative (if present), and the ICU and ward nurses who were involved in the transition process.
Pyrolysis of lignocellulosic biomass and waste plastics has been intensely studied in the last few decades to obtain renewable fuels and chemicals. Various pyrolysis devices have been developed for use in a laboratory setting, operated either in batch or continuously at scales ranging from milligrams per hour to tenths of g per hour. We report here the design and operation of a novel staged free-fall (catalytic) pyrolysis unit and demonstrate that the concept works very well for the (catalytic) pyrolysis of pinewood sawdust, paper sludge, and polypropylene as representative feeds.
View Article and Find Full Text PDFSubunit vaccines would benefit from a safe particle-based adjuvant. Elastin-like polypeptide (ELP)-based micelles are interesting candidate adjuvants due to their well-defined size and easy modification with protein-based cargo. Coiled coils can facilitate noncovalent modifications, while potentially enhancing antigen delivery through interaction with cell membranes.
View Article and Find Full Text PDFQuorum sensing (QS) serves as a vital means of intercellular signalling in a variety of prokaryotes, which enables single cells to act in multicellular configurations. The potential to control community-wide responses has also sparked numerous recent biotechnological innovations. However, our capacity to utilize intercellular communication is hindered due to a scarcity of complementary signalling systems and a restricted comprehension of interconnections between these systems caused by variations in their dynamic range.
View Article and Find Full Text PDFComplexome profiling allows large-scale, untargeted, and comprehensive characterization of protein complexes in a biological sample using a combined approach of separating intact protein complexes e.g., by native gel electrophoresis, followed by mass spectrometric analysis of the proteins in the resulting fractions.
View Article and Find Full Text PDFAlthough aluminum hydroxide (alum) is widely accepted and used as safe vaccine adjuvant, there is some concern about possible toxicity upon long-lasting repeated exposure during subcutaneous allergen immunotherapy (SCIT). Our objective was to evaluate allergen-bearing liposomes as possible alternative for alum-adsorption in SCIT. A self-assembling, coiled-coil forming peptide pair was used to anchor the major birch pollen allergen Bet v 1 to the surface of cationic liposomes.
View Article and Find Full Text PDFNew and improved nanomaterials are constantly being developed for biomedical purposes. Nanomaterials based on elastin-like polypeptides (ELPs) have increasingly shown potential over the past two decades. These polymers are artificial proteins of which the design is based on human tropoelastin.
View Article and Find Full Text PDFPrevious event-related potential (ERP) studies have shown that snake pictures elicit greater early posterior negativity (EPN) compared to other animal pictures. The EPN reflects early selective visual processing of emotionally significant stimuli. Evidence for the role that high and low spatial frequencies play in the early detection of snakes is still inconsistent.
View Article and Find Full Text PDFThere is growing concern about the toxicity of colloidal aluminum salts used as adjuvants in subcutaneous allergen immunotherapy (SCIT). Therefore, alternative adjuvants and delivery systems are being explored to replace alum in SCIT. We applied micellar elastin-like polypeptides (ELPs), a type of self-assembling protein, to replace alum as vaccine adjuvant in birch pollen SCIT.
View Article and Find Full Text PDFBackground: Optimising intranasal distribution and retention of topical therapy is essential for effectively managing patients with chronic rhinosinusitis, including those that have had functional endoscopic sinus surgery (FESS). This study presents a new technique for quantifying in vitro experiments of fluticasone propionate deposition within the sinuses of a 3D-printed model from a post-FESS patient.
Methods: Circular filter papers were placed on the sinus surfaces of the model.
So-called ρ0 cells lack mitochondrial DNA and are therefore incapable of aerobic ATP synthesis. How cells adapt to survive ablation of oxidative phosphorylation remains poorly understood. Complexome profiling analysis of ρ0 cells covered 1,002 mitochondrial proteins and revealed changes in abundance and organization of numerous multiprotein complexes including previously not described assemblies.
View Article and Find Full Text PDFThe dual-process theory is a significant theory in both organizational theory and social psychology and two conjectures about this theory are considered in this manuscript; the default-interventionist vs. parallel-competitive account. Our research goal is to empirically investigate these two views.
View Article and Find Full Text PDFspecies have a single mitochondrion that is essential for their survival and has been successfully targeted by antimalarial drugs. Most mitochondrial proteins are imported into this organelle, and our picture of the mitochondrial proteome remains incomplete. Many data sources contain information about mitochondrial localization, including proteome and gene expression profiles, orthology to mitochondrial proteins from other species, coevolutionary relationships, and amino acid sequences, each with different coverage and reliability.
View Article and Find Full Text PDFBiochim Biophys Acta Bioenerg
July 2021
Complexome profiling is an emerging 'omics' approach that systematically interrogates the composition of protein complexes (the complexome) of a sample, by combining biochemical separation of native protein complexes with mass-spectrometry based quantitation proteomics. The resulting fractionation profiles hold comprehensive information on the abundance and composition of the complexome, and have a high potential for reuse by experimental and computational researchers. However, the lack of a central resource that provides access to these data, reported with adequate descriptions and an analysis tool, has limited their reuse.
View Article and Find Full Text PDFSnakes have proven to drive early attentional capture due to their evolutionary importance, as reflected by the early posterior negativity (EPN). The EPN snake effect might be partly driven by the proximity of the animal. In this study, by employing full-body (medium shot) and head-focused (close-up) pictures, we investigated whether the relative nearness (proximity) of the animal on the picture affects the snake EPN effect.
View Article and Find Full Text PDFThe primary objective of this research was to extract the essential information needed for setting atomization break up models, specifically, the Linear Instability Sheet Atomization (LISA) breakup model, and alternative hollow cone models. A secondary objective was to gain visualization and insight into the atomization break up mechanism caused by the effects of viscosity and surface tension on primary break-up, sheet disintegration, ligament and droplet formation. High speed imaging was used to capture the near-nozzle characteristics for water and drug formulations.
View Article and Find Full Text PDFFacial expressions affect memory for face identity. We tested how fearful expressions modulate recognition memory for faces. In two studies, participants completed a continuous recognition task with fearful and neutral faces while their electroencephalogram was recorded.
View Article and Find Full Text PDFProtein complexes from the oxidative phosphorylation (OXPHOS) system are assembled with the help of proteins called assembly factors. We here delineate the function of the inner mitochondrial membrane protein TMEM70, in which mutations have been linked to OXPHOS deficiencies, using a combination of BioID, complexome profiling and coevolution analyses. TMEM70 interacts with complex I and V and for both complexes the loss of TMEM70 results in the accumulation of an assembly intermediate followed by a reduction of the next assembly intermediate in the pathway.
View Article and Find Full Text PDFInt J Psychophysiol
December 2019
Previous event-related potential studies have reported enhanced Early Posterior Negativity (EPN) in response to snake pictures compared to pictures of other animals. This EPN snake effect may be partly driven by specific snake skin patterns. In this study, by using blurred pictures to make these patterns less visible, we explored whether the relative absence of such local features will attenuate the EPN snake effect.
View Article and Find Full Text PDFThe beloved likely attracts attention. We tested whether the beloved captures early automatic attention, as indicated by an early posterior negativity (EPN) in a typical rapid serial visual presentation (RSVP) task, while replicating previous findings that the beloved receives sustained motivated attention, as indicated by an enhanced late positive potential (LPP) in a standard passive viewing task. Participants viewed beloved, friend, and stranger pictures in RSVP and standard passive viewing tasks.
View Article and Find Full Text PDFGiven the importance of risk-taking in individuals' personal and professional life, several behavioral tasks for measuring the construct have been developed. Recently, a new task was introduced, the Columbia Card Task (CCT). This task measures participants' risk levels and establishes how sensitive participants are to gains, losses, and probabilities when taking risk.
View Article and Find Full Text PDFMotivation: Complexome profiling combines native gel electrophoresis with mass spectrometry to obtain the inventory, composition and abundance of multiprotein assemblies in an organelle. Applying complexome profiling to determine the effect of a mutation on protein complexes requires separating technical and biological variations from the variations caused by that mutation.
Results: We have developed the COmplexome Profiling ALignment (COPAL) tool that aligns multiple complexome profiles with each other.
Previous studies have examined the role of the eye region in emotional expressions, but the mouth region is understudied. The main goal of this study was to examine how mouth opening in emotional faces affects subjective experience and early automatic attentional capture, as measured by the early posterior negativity (EPN) amplitude. Participants in two studies viewed angry, happy, and neutral faces with mouths open and closed while their electroencephalogram was recorded.
View Article and Find Full Text PDF