Spirulina platensis as renewable accumulator for heavy metals accumulation from multi-element synthetic effluents.

Metal accumulation by Spirulina platensis from synthetic effluents with the following chemical composition: Cr/Fe, Cr/Fe/Ni, Cr/Fe/Ni/Zn, and Cr/Fe/Ni/Zn/Cu during repeated cultivation cycle was investigated. Metal ions in different concentrations were added to the culture medium at the exponential and stationary phases of biomass growth and their uptake by biomass was traced using neutron activation analysis. The effect of metal ions on biomass and main biochemical components (proteins, carbohydrates, lipids, phycobilins, and β-carotene) was monitored.

Heavy metal contamination of topsoils around a lead and zinc smelter in the Republic of Macedonia.

The results of a first systematic study of spatial distribution of different elements in surface soil over the Veles region (50 km(2)) known for its lead and zinc industrial activity in the recent past are reported. A total of 201 soil samples were collected according to a dense net in urban area and less dense net in rural area. The total of 42 elements were analyzed by epithermal neutron activation analysis (ENAA) and by atomic absorption spectrometry (AAS).

[Different assays for hingamine in the study of biological fluids].

The authors present a method of hingamine identification in non-biological substances (tablets, powder, syringes) and biological fluids (blood, urine). Isolation was made with chloroform in pH 10. Identification was conducted with thin-layer chromatography, gas chromatography/mass-spectrometry, high-performance liquid chromatography, spectrophotometry in UV region.

[Estimation of various quantitative methods for the determination of native and formaldehyde-treated proteins].

Quantitative estimation of native and treated with 4% formaldehyde albumin, pepsin, lysozyme, histone, gelatin and other proteins was carried out using four procedures--biurete, Lowry-Folin, ninhydrin and coomassy R-250. Chromogeneity of proteins in corresponding color reactions was expressed as OD per 100 micrograms of nitrogen estimated by means of Kjeldahl micromethod. The proteins were treated with formaldehyde in corresponding buffers at 20 degrees within 7 days, non-bound or loosely bound formaldehyde was dialysed.