Rational fragment-based design of compounds targeting the PWWP domain of the HRP family.

Lens epithelium-derived growth factor p75 (LEDGF/p75), member of the hepatoma-derived growth-factor-related protein (HRP) family, is a transcriptional co-activator and involved in several pathologies including HIV infection and malignancies such as MLL-rearranged leukemia. LEDGF/p75 acts by tethering proteins to the chromatin through its integrase binding domain. This chromatin interaction occurs between the PWWP domain of LEDGF/p75 and nucleosomes carrying a di- or trimethylation mark on histone H3 Lys36 (H3K36me2/3).

Calibrated SoilOptix ® estimates of soil pH and exchangeable cations in three agricultural fields in western Canada - implications for managing spatially variable soil acidity.

Spatial variability in soil pH is a major contributor to within-field variations in soil fertility and crop productivity. An improved understanding of the spatial variability of soil pH within agricultural fields is required to determine liming requirements for precision farming. This study with the use of proximal sensors, firstly assessed the spatial pattern of soil pH and how it can be used to determine site-specific, spatially variable lime requirements.

Optimizing Clubroot Management and the Role of Canola Cultivar Mixtures.

The sustainable cultivation of canola is under threat from clubroot disease (). The pathogen's resting spores can survive in the soil for extended periods, complicating disease management. Therefore, effective clubroot control requires a combination of tactics that provide multiple layers of protection.

Clubroot-Induced Changes in the Root and Rhizosphere Microbiome of Susceptible and Resistant Canola.

Clubroot is a soilborne disease of canola () and other crucifers caused by the obligate parasite . In western Canada, clubroot is usually managed by planting-resistant cultivars, but the emergence of resistance-breaking pathotypes of represents a major threat to sustainable canola production. The rhizosphere and root contain beneficial microorganisms that can improve plant health.

The Host Range of in Western Canada.

is associated with the root rot of many plant species, but knowledge of its impact on western Canadian field crops is limited. This study assessed the host range of this fungus and its effect on plant emergence, plant height, and shoot and root dry weights in repeated greenhouse experiments with wheat, barley, faba beans, peas, lentils, canola, lupine, and soybeans. Infection was confirmed via PCR, and principal component analysis determined the utility of different parameters in assessing host responses.

Pathogenicity, Host Resistance, and Genetic Diversity of Species under Controlled Conditions from Soybean in Canada.

spp. are commonly associated with the root rot complex of soybean (). Previous surveys identified six common species from Manitoba, including , , , , , and .

Identification of Clubroot () Resistance Loci in Chinese Cabbage ( ssp. ) with Recessive Character.

The soil-borne pathogen is the causal agent of clubroot, a major disease in Chinese cabbage ( ssp. ). The host's resistance genes often confer immunity to only specific pathotypes and may be rapidly overcome.

A high-throughput target-based screening approach for the identification and assessment of mycothione reductase inhibitors.

Unlabelled: The World Health Organization's goal to combat tuberculosis (TB) is hindered by the emergence of anti-microbial resistance, therefore necessitating the exploration of new drug targets. Multidrug regimens are indispensable in TB therapy as they provide synergetic bactericidal effects, shorten treatment duration, and reduce the risk of resistance development. The research within our European RespiriTB consortium explores energy metabolism to identify new drug candidates that synergize with bedaquiline, with the aim of discovering more efficient combination drug regimens.

Experience in the Application of Augmented Reality Technology in the Surgical Treatment of Patients Suffering Primary and Recurrent Pelvic Tumors.

Surgical treatment of locally spread tumors in pelvic organs remains an urgent and complicated oncological problem. The recurrence rate after radical treatment ranges from 15.1% to 45.

Evaluation of Amisulbrom Products for the Management of Clubroot of Canola ().

Clubroot, caused by , is an important disease of canola (). Amisulbrom, a quinone inside inhibitor (QiI), was evaluated for its effectiveness in clubroot management in Alberta, Canada. Resting spores of were treated in vitro with 0, 0.

Impact of Susceptibility on Plant Hormonal Composition during Clubroot Disease Development in Canola ().

Clubroot, caused by , is a soilborne disease of crucifers associated with the formation of large root galls. This root enlargement suggests modulation of plant hormonal networks by the pathogen, stimulating cell division and elongation and influencing host defense. We studied physiological changes in two cultivars, including plant hormone profiles-salicylic acid (SA), jasmonic acid (JA), abscisic acid (ABA), the auxin indole-3-acetic acid (IAA), and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC)-along with their selected derivatives following inoculation with virulent and avirulent pathotypes.

A Hydroponic-Based Bioassay to Facilitate Phenotyping.

Clubroot, caused by the obligate parasite , is one of the most devastating diseases affecting the canola/oilseed rape () industry worldwide. Currently, the planting of clubroot-resistant (CR) cultivars is the most effective strategy used to restrict the spread and the economic losses linked to the disease. However, virulent isolates have been able to infect many of the currently available CR cultivars, and the options to manage the disease are becoming limited.

Molecular structure of soluble vimentin tetramers.

Intermediate filaments (IFs) are essential constituents of the metazoan cytoskeleton. A vast family of cytoplasmic IF proteins are capable of self-assembly from soluble tetrameric species into typical 10-12 nm wide filaments. The primary structure of these proteins includes the signature central 'rod' domain of ~ 300 residues which forms a dimeric α-helical coiled coil composed of three segments (coil1A, coil1B and coil2) interconnected by non-helical, flexible linkers (L1 and L12).

Effect of Plasma-Activated Water Bubbles on , Deoxynivalenol, and Germination of Naturally Infected Barley during Steeping.

Contamination of barley by deoxynivalenol (DON), a mycotoxin produced by , causes considerable financial loss to the grain and malting industries. In this study, two atmospheric cold plasma (ACP) reactors were used to produce plasma-activated water (PAW) bubbles. The potential of PAW bubbles for the steeping of naturally infected barley (NIB) during the malting process was investigated.

A Revised Nomenclature for Haplotypes Across Multiple Fungal Species.

ToxA is one of the most studied proteinaceous necrotrophic effectors produced by plant pathogens. It has been identified in four pathogens (, , [formerly f. sp.

Blackleg Yield Losses and Interactions with Verticillium Stripe in Canola () in Canada.

Blackleg, caused by , is an important disease of canola (). The pathogen can attack stems, leaves and pods, but basal stem cankers are most damaging and can result in significant yield losses. In Canada, Verticillium stripe () has recently emerged as another disease threat to canola.