Clonal genomic population structure of Beauveria brongniartii and Beauveria pseudobassiana: Pathogens of the common European cockchafer (Melolontha melolontha L.).

Beauveria brongniartii is a fungal pathogen that infects the beetle Melolontha melolontha, a significant agricultural pest in Europe. While research has primarily focused on the use of B. brongniartii for controlling M.

Spatial and temporal patterns in the population genomics of the European cockchafer in the Alpine region.

The European cockchafer is an agricultural pest in many European countries. Populations have a synchronized 3 or 4 years life cycle, leading to temporally isolated populations. Despite the economic importance and availability of comprehensive historical as well as current records on cockchafer occurrence, population genomic analyses of are missing.

Light in the box-photobiological examination chamber with light trap ventilation system for studying fungal surface cultures illustrated with Metarhizium brunneum and Beauveria brongniartii.

Due to their versatile way of life as saprophytes, endophytes, and entomopathogens, fungi of the genera Metarhizium and Beauveria are exposed to varying illumination conditions in their natural habitats, which makes a thorough adaptation to light very likely. While the few available studies for these genera support this assumption, research in this field is still in its infancy and the data material restricted to only a few fungal species. Thus, the aim of this work was to explore how light influences growth, conidial production and secondary metabolite formation of two industrial relevant strains of M.

Integrated Biological Control of the Sugar Beet Weevil with the Fungus : New Application Approaches.

The mass occurrence of the sugar beet weevil (Asproparthenis punctiventris, previously Bothynoderes punctiventris) has been endangering sugar beet cultivation in Austria for centuries. Exacerbated by climatic and political changes (warmer, drier spring and limited access to chemical pesticides), new approaches are needed to counter the problem. The aim of our work was to test whether the bioinsecticide Metarhizium brunneum Ma 43 (formerly M.

Development of a SNP-based tool for the identification and discrimination of and .

The European (Melolontha melolontha L.) and Forest (M. hippocastani F.

[Addictions].

This article describes a new form of administration of inhaled prescribed heroin currently under investigation. It underlines the particularity of opioid agonist prescription in jail and presents new perspectives in using psilocybin in addiction medicine treatment. A brief literature review about vaping confirms its interest in quitting cigarette, with a cost of addiction to vaping and recent worrisome reports of chemical pneumonia.

Analytical Methods for Secondary Metabolite Detection.

The entomopathogenic fungi Metarhizium brunneum, Beauveria bassiana, and B. brongniartii are widely applied as biological pest control agent in OECD countries. Consequently, their use has to be flanked by a risk management approach, which includes the need to monitor the fate of their relevant toxic metabolites.

Audiometric studies versus predictions of drastic deteriorations of hearing protectors' noise attenuation due to reduced wearing time: Misjudgments by standards based on the energy-equivalence principle.

Background: International standards based on the energy-equivalence principle predict a drastic deterioration of hearing protectors' noise attenuation if the protector is not worn for only brief time periods.

Objective: Therefore, studies in a laboratory setting were carried out to examine whether corresponding temporary threshold shifts (TTS) due to realistic noise exposures can be found.

Methods: Ten test subjects (Ss) were exposed to two reference exposures of 94 dB (A) for 1 h and 97 dB (A) for 1/2 h.

Supercritical Fluid Chromatography as an Alternative Tool for the Qualitative and Quantitative Analysis of Metarhizium brunneum Metabolites from Culture Broth.

A fast and selective ultrahigh-performance supercritical fluid chromatography photodiode array detector method was established for the qualitative and quantitative analysis of destruxins, cyclic hexadepsipeptides, from fungal culture broth samples. Prior to analysis, sample purification was carried out using an off-line solid-phase extraction protocol on a reversed-phase material in order to remove unwanted matrix constituents. For separation, detection, and identification, an ultrahigh-performance supercritical fluid chromatography photodiode array detector system hyphenated to a triple quadrupole mass spectrometer was utilized.

Quantitative Assessment of Destruxins from Strawberry and Maize in the Lower Parts per Billion Range: Combination of a QuEChERS-Based Extraction Protocol with a Fast and Selective UHPLC-QTOF-MS Assay.

The entomopathogenic fungus Metarhizium brunneum is widely applied as a biological pest control agent. Consequently, its use has to be accompanied by a risk management approach, which includes the need to monitor the fate of its bioactive metabolites in the environment, for example, in treated crops. A fast and selective UHPLC-QTOF-MS method was developed to monitor the presence of secreted destruxins in two model food plants for the application of this fungal biocontrol agent, namely, strawberry and maize.

Thermal Inactivation of Shiga Toxin-Producing Escherichia coli Cells within Cubed Beef Steaks following Cooking on a Griddle.

Thermal inactivation of Shiga toxin-producing Escherichia coli (STEC) cells within knitted/cubed beef steaks following cooking on a nonstick griddle was quantified. Both faces of each beef cutlet (ca. 64 g; ca.

Testosterone Replacement Therapy in Hypogonadal Men Following Prostate Cancer Treatment: A Questionnaire-Based Retrospective Study among Urologists in Bavaria, Germany.

Background: Several reports suggest testosterone replacement therapy (TRT) may be an option in selected hypogonadal patients with a history of prostate cancer (PCa) and no evidence of disease after curative treatment. Our aim was to assess TRT experience and patient management among urologists in Bavaria.

Materials And Methods: Questionnaires were developed and mailed to all registered urologists in Bavaria (n = 420) regarding their experience with TRT in patients with treated PCa.

Development of a fast and selective UHPLC-DAD-QTOF-MS/MS method for the qualitative and quantitative assessment of destruxin profiles.

A fast and selective ultrahigh-performance liquid chromatography diode array detector (UHPLC-DAD) method combined with an off-line solid phase extraction (SPE) protocol was established to monitor destruxins (dtxs), a secondary metabolite class of highly bioactive cyclic depsipeptides. Sample purification via SPE was tailored to remove both more polar and apolar matrix constituents by applying analyte class-selective washing and elution conditions. To separate and detect destruxin congeners an UHPLC-DAD system hyphenated to a quadrupole-time-of-flight (Q-TOF) hybrid mass spectrometer was utilized.

Cannabis use among a sample of 16 to 18 year-old students in Switzerland.

Background: The aim of this study was to estimate the prevalence of cannabis use among Swiss students and to assess their attitudes regarding health and safety issues associated with drug use.

Subjects And Methods: After a workshop, 173 students (23.1% male, 75.

Order-picking in deep cold--physiological responses of younger and older females. Part 2: body core temperature and skin surface temperature.

So far, it was unclear to what extent working in deep cold-storage depots has an influence on female order-pickers body core temperature and skin surface temperature considering different age groups. Physiological effects of order-picking in a chill room (+3°C) and cold store (-24°C) were examined on 30 female subjects (Ss), classified in two age groups (20- to 35- year-olds and 40- to 65-year-olds). The body core temperature was taken every 15 min at the tympanum and the skin surface temperature was recorded continuously at seven different positions.

Order-picking in deep cold--physiological responses of younger and older females. Part 1: heart rate.

The sales figures of chilled and frozen food have been rising steadily over the years. Naturally, this has also led to an increase in the number of jobs related to these goods. While these workplaces are becoming more and more important there are, nevertheless, only a few investigations into the effects of working in deep cold on humans.

Carbon utilization pattern as a potential quality control criterion for virulence of Beauveria brongniartii.

The registered entomopathogenic fungus Beauveria brongniartii (BIPESCO 2) was tested for its virulence after one, five and 10 times sub-culturing on four types of selective synthetic nutrient media. Bioassays with third instar Melolontha melolontha larvae showed that sub-culturing negatively affects the virulence of the fungus after 10 transfers. With the Biolog SF-P2 and Biolog SF-N2 microtiter plate systems the sub-cultivated B.

Formation of exudate droplets by Metarhizium anisopliae and the presence of destruxins.

Nutritional conditions causing droplet exudation by Metarhizium anisopliae var. anisopliae were studied. Exudation in droplets occurred only on media with more than one carbon source and was highly dependent on the ratio of a well metabolized sugar such as trehalose and a nonpreferred sugar, in particular arabinose.

Destruxin production of Metarhizium anisopliae under carbon and nitrogen exhaustion.

Destruxins (dtx) A, B, and E, showing a variety of biological activities, are the main toxic secondary metabolites of the entomopathogenous ascomycete Metarhizium anisopliae Bipesco 5, a widely used biocontrol production strain. Dynamics of dtx biosynthesis were monitored during liquid fermentation in a chemically defined medium. During shake flask cultivation with excess carbon, nitrogen and phosphate, approximately 50, 20, and 100 mg l(-1) dtx A, B, and E were produced after 12 d.

Can autologous myoblasts be used as a potential bulking agent?

Objective: To investigate the behaviour of donor myoblasts at the vesico-ureteric junction (VUJ) and to evaluate their potential as an autologous bulking agent, as myoblast transplantation has been shown to regenerate damaged or degenerated tissue, and it was postulated that they could be used to treat vesico-ureteric reflux.

Materials And Methods: Muscle biopsies were obtained from the lower limb muscles of 10 pigs. The quality of the cells was evaluated by electrophysiological and immunohistochemical tests.

Algorithm-based design of synthetic growth media stimulating virulence properties of Metarhizium anisopliae conidia.

Aims: Synthetic media should be designed for the production of Metarhizium anisopliae conidia with improved virulence properties.

Methods And Results: A genetic algorithm (GA), demonstrated to be suitable for the design of media for spore mass production (Hutwimmer et al. 2008), was utilized for a multi-objective medium design to improve conidia yield and three proposed virulence properties of conidia: C : N ratio, germination speed and amount of spore-bound Pr1 protease.