In sito galanthamine extraction during the cultivation of L. shoot culture in two-phase bubble column cultivation system.

Two-phase bioreactor cultivation system was developed and applied for in sito recovery of extracellular galanthamine during the cultivation of L. shoot culture in a modified column bioreactor system. The inclusion of an external circulation column with adsorbent resin Amberlite XAD-4 as a second phase, on the 21st day of the beginning of cultivation resulted in 1.