To improve the understanding of the extraction chemistry of An(iii) and Ln(iii) with N-donor ligands 2,6-bis(5-(-butyl)-1-pyrazol-3-yl)pyridine (C4-BPP) in the presence of 2-bromohexanoic acid was investigated. Extraction studies showed an excellent separation factor of SF ≈ 200 and SF ≈ 60 in comparison with the structurally similar ligand 2,6-bis(5-neopentyl-1-pyrazol-3-yl)pyridine C5-BPP (SF ≈ 100), even though C5-BPP showed significantly higher stability constants. Time-resolved laser fluorescence spectroscopy (TRLFS) studies revealed the formation of the ternary 1 : 1 and 1 : 2 complexes [Eu(C4-BPP) (2-bromohexanoate) ] ( = 1-2) ( and ).
View Article and Find Full Text PDFInnovative analytical instruments and development of new methods has provided a better understanding of protein particle formation in biopharmaceuticals but have also challenged the ability to obtain reproducible and reliable measurements. The need for protein-like particle standards mimicking the irregular shape, translucent nature and near-to-neutral buoyancy of protein particles remained one of the hot topics in the field of particle detection and characterization in biopharmaceutical formulations. An innovative protein-like particle model has been developed using two photo polymerization (2PP) printing allowing to fabricate irregularly shaped particles with similar properties as protein particles at precise size of 50 µm and 150 µm, representative of subvisible particles and visible particles, respectively.
View Article and Find Full Text PDFLike other members of the species Gallus gallus, fast-growing broilers are motivated to perch. However, broilers in the European Union are kept in unstructured barns, with no opportunity to sit elevated and rest undisturbed. A possible solution to this problem is elevated platforms.
View Article and Find Full Text PDFAntibody combination therapies have become viable therapeutic treatment options for certain severe diseases such as cancer. The co-formulation production approach is intrinsically associated with more complex drug product variant profiles and creates more challenges for analytical control of drug product quality. In addition to various individual quality attributes, those arising from the interactions between the antibodies also potentially emerge through co-formulation.
View Article and Find Full Text PDFInjurious pecking against conspecifics is a serious problem in turkey husbandry. Bloody injuries act as a trigger mechanism to induce further pecking, and timely detection and intervention can prevent massive animal welfare impairments and costly losses. Thus, the overarching aim is to develop a camera-based system to monitor the flock and detect injuries using neural networks.
View Article and Find Full Text PDFFootpad dermatitis (FPD) is an indicator of animal welfare in turkeys, giving evidence of the animals' physical integrity and providing information on husbandry management. Automated systems for assessing FPD at slaughter can present a useful tool for objective data collection. However, using automated systems requires that they reliably assess the incidence.
View Article and Find Full Text PDFUnlabelled: Living donor robotic-assisted kidney transplantation (RAKT) is an alternative to open kidney transplantation (OKT), but experience with this technique is limited in the United States.
Methods: A retrospective review of living donor kidney transplants performed between 2016 and 2018 compared RAKT with OKT with regard to recipient, donor, and perioperative parameters. A 1:1 propensity score matching was performed on recipient/donor age, sex, body mass index, race, preoperative dialysis, and calculated panel reactive antibodies.
Broiler chickens in Europe are usually raised in a barren environment. Elevated perforated platforms address this problem and can positively influence animal health and welfare. To evaluate the effect of an elevated perforated floor on the behavior of broiler chickens, one of two barns was equipped with a perforated flooring system under the food and water supply.
View Article and Find Full Text PDFThis study aimed to develop a camera-based system using artificial intelligence for automated detection of pecking injuries in turkeys. Videos were recorded and split into individual images for further processing. Using specifically developed software, the injuries visible on these images were marked by humans, and a neural network was trained with these annotations.
View Article and Find Full Text PDFObjective: To describe the use of robotic-assisted transplant ureteral repair (RATUR) for treating transplant ureteral stricture (TUS) in 3 patients who had undergone robot assisted kidney transplant (RAKT).
Method: We reviewed the medical records of 3 patients who experienced TUS after RAKT and who underwent RATUR between 2017 and 2020. The patients' RAKT, post-transplant clinical course, endourological interventions, reoperation, and recovery were assessed.
Stabilization of critical reagents by freeze-drying would facilitate storage and transportation at ambient temperatures, and simultaneously enable constant reagent performance for long-term bioanalytical support throughout drug development. Freeze-drying as a generic process for stable performance and storage of critical reagents was investigated by establishing an universal formulation buffer and lyophilization process. Using a storage-labile model protein, formulation buffers were evaluated to preserve reagent integrity during the freeze-drying process, and to retain functional performance after temperature stress.
View Article and Find Full Text PDFClaw lesions are a serious problem on dairy farms, affecting both the health and welfare of the cow. Automated detection of lameness with a practical, on-farm application would support the early detection and treatment of lame cows, potentially reducing the number and severity of claw lesions. Therefore, in this study, a method was proposed for the detection of claw lesions based on the acoustic analysis of a cow's gait.
View Article and Find Full Text PDFOxidative damage of biopharmaceuticals during manufacturing and storage is a key concern throughout pharmaceutical development. However, few simple and robust analytical methods are available for the determination of oxidation sites. Here, the potential of affinity capillary electrophoresis (ACE) in the separation of proteins with oxidized methionine (Met) residues is shown.
View Article and Find Full Text PDFAnimal welfare is one of the most challenging issues in modern farm animal husbandry. Animal welfare indicators can be used to monitor welfare on farms or at slaughterhouses, with footpad dermatitis (FPD) being one of the most important indicators used in turkeys. Up to now, the severity of FPD has been measured by evaluating the size of altered lesions on the metatarsal pad of birds.
View Article and Find Full Text PDFIn this study, a new housing system for broiler was tested. This system consisted of a slatted floor area and a littered area with the aim of improving litter quality. Two experimental broiler houses were provided.
View Article and Find Full Text PDFThis study investigates air hygienic characteristics of housing systems without fixation for lactating sows, with a focus on microbiological air quality. For animal welfare considerations, free-farrowing systems have been developed to replace those with farrowing crates (FC) and are currently available on the market. A group housing system (GH) for six lactating sows, a single loose-housing system (LH), and a conventional system with FC were tested.
View Article and Find Full Text PDFThe assessment of bird-based welfare indicators plays an important role in the evaluation of bird welfare. The aim of the study was to histologically validate a visual scoring system for hock burn in broilers and to detect threshold values of a visual score to define welfare-relevant alterations in terms of mild lesions or ulcers of the hock. We collected 200 hocks of 39- to 42-day-old Ross 308 broilers after the slaughter process.
View Article and Find Full Text PDFObjectives: During a 'cannot intubate, cannot oxygenate' situation, asphyxia can lead to cardiac arrest. In this stressful situation, two complex algorithms facilitate decision-making to save a patient's life: difficult airway management and cardiopulmonary resuscitation. However, the extent to which competition between the two algorithms causes conflicts in the execution of pivotal treatment remains unknown.
View Article and Find Full Text PDFWet litter is the most important cause of footpad dermatitis in poultry, this in turn being a highly relevant animal-related welfare indicator. This field study was subdivided into two experiments. In Experiment 1, the standard diet was supplemented by 0.
View Article and Find Full Text PDFThe aim of the presented study was to validate a three-point locomotion score (LS) classifying lameness in dairy cows. Therefore, locomotion of 144 cows was scored and data on claw lesions were collected during hoof trimming. Based on latter data a cluster analysis was performed to objectively classify cows into three groups (Cluster 1-3).
View Article and Find Full Text PDFMeasuring the severity of foot-pad dermatitis is an accepted tool monitoring the quality of animal husbandry and welfare. Up to now, a variety of scoring schemes have been used, most of them based on visual evaluation. However, a standardisation and validation of scoring systems is beneficial, not only to compare different studies, but to provide objective indicators in poultry welfare.
View Article and Find Full Text PDFCZE is a well-established technique for charge heterogeneity testing of biopharmaceuticals. It is based on the differences between the ratios of net charge and hydrodynamic radius. In an extensive intercompany study, it was recently shown that CZE is very robust and can be easily implemented in labs that did not perform it before.
View Article and Find Full Text PDFCognitive and affective processes are highly interrelated. This has implications for neuropsychiatric disorders such as major depressive disorder in humans but also for the welfare of non-human animals. The brain serotonergic system might play a key role in mediating the relationship between cognitive functions and affective regulation.
View Article and Find Full Text PDFElectrophoresis
March 2017
During the last years there was a substantial increase in the use of antibodies and related proteins as therapeutics. The emphasis of the pharmaceutical industry is on IgG1, IgG2, and IgG4 antibodies, which are therefore in the focus of this article. In order to ensure appropriate quality control of such biopharmaceuticals, deep understanding of their chemical degradation pathways and the resulting impact on potency, pharmacokinetics, and safety is required.
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