Publications by authors named "Stoute S"

Article Synopsis
  • In a recent study covering cases from 2014 to 2023, sodium toxicosis in chickens was linked to serious health issues and significant losses in the poultry industry, with a detailed analysis conducted on 7 affected cases.
  • The autopsy findings revealed common lesions including fluid accumulation in various organs, respiratory and kidney issues, and significant differences in sodium levels in the brains of affected versus healthy chickens.
  • Feed samples from sodium toxicosis cases showed sodium levels far exceeding the safe limit of 2,000 ppm, ranging from 2,500 to 12,000 ppm, indicating a possible cause for the toxicity observed in affected chickens.
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Low pathogenicity avian influenza (LPAI) H7N3 was diagnosed in a flock of commercially raised Japanese quail () breeder hens. The birds were submitted with an initial complaint of a drop in egg production and watery droppings. Initial PCR testing of the flock for avian influenza (AI) was negative; however, mortality started increasing drastically, prompting further examination and the submission of more birds to the diagnostic laboratory.

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has been associated with spontaneous and antibiotic-associated enteric disease (associated enteric disease, CSAED) in rabbits, which is clinically characterized by anorexia, diarrhea, or sudden death. Diagnosis is usually based on gross and microscopic lesions, coupled with finding the characteristic coiled bacteria in intestinal smears. Isolation of is often challenging, and a PCR protocol has been developed.

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Background: Abuse of healthcare workers (HCWs) and lack of public trust threaten the foundation of the physician-patient relationship. This growing global problem creates an even more difficult professional environment and hinders the delivery of high-quality clinical care.

Objective: The primary aim was to determine the prevalence of violence against Trinbagonian HCWs in the public sector.

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Highly pathogenic avian influenza (HPAI) has resulted in catastrophic economic losses globally in poultry. This case report describes the diagnostic detection and pathology of HPAI H5N1 in 5-day-old commercial ducklings, which is an atypical age for detection of natural infection of HPAI in poultry. The pathology observed at 5 days of age was also compared to lesions observed in ducklings from the same flock evaluated at 10 days of age before depopulation.

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Herein, we report the complete genome for an avian infectious bronchitis virus isolated from cecal tonsils of California layers in 2021. This whole-genome sequence belongings to genotype GVIII, previously classified as a unique variant.

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Article Synopsis
  • False layer syndrome affects chicks by causing infections with the infectious bronchitis virus (IBV), leading to permanent damage to their oviducts and resulting in reduced or no egg production.
  • A study of a commercial ISA Brown layer flock revealed signs of this syndrome, including cystic oviducts and abnormal egg production, through diagnostics on 31 submitted birds.
  • Identification of IBV involved sequencing that showed a 97% similarity to the California variant CA1737/04, but further studies are needed to definitively prove its link to reproductive issues.
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  • A 4-month-old female mixed-breed chicken showed chronic weight loss and gastro issues before being euthanized and submitted for postmortem analysis.
  • The examination revealed significant gastrointestinal problems, including severe impaction and necrosis, with presence of white nodules and eroded gizzard lining.
  • The cause of these issues was linked to an overgrowth of bacteria due to a diet of fibrous plant material and grain, marking a unique case of gastrointestinal impaction associated with necrotic enteritis in backyard chickens.
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We characterized naturally occurring pigeon herpesvirus (PiHV; ) infection in domestic pigeons in California. We retrieved and analyzed 62 pathology reports produced between 1991 and 2014 at the California Animal Health and Food Safety Laboratory System. In 56 of the cases, the diagnosis of PiHV infection was established based on histopathology, either alone (44 cases) or combined with virus isolation (VI; 8), transmission electron microscopy (TEM; 3), or PCR (1); in the remaining 6 cases, the diagnosis was established based on VI (5 cases) or TEM (1) alone.

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Four turkeys from a commercial flock with acutely elevated mortality levels were submitted for postmortem examination and diagnostic workup. No clinical signs had been observed before death. On gross examination, hemorrhage and necrosis were present throughout the intestinal tracts, and the spleens were markedly enlarged and speckled.

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Infectious bronchitis virus (IBV) causes severe economic losses among chicken flocks worldwide. Although IBV molecular surveillance has been conducted in California broilers, seasonal and spatial-temporal trends in IBV prevalence are poorly defined. The goals of this study were to evaluate seasonal and spatial-temporal trends in IBV prevalence and to determine the predominant IBV genotypes obtained over the last 8 yr from a broiler company located in the California Central Valley.

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Article Synopsis
  • - Infectious laryngotracheitis (ILT) is a significant respiratory disease in chickens, and a study analyzed gross and histological lesions to assess diagnostic test sensitivity across various chicken flocks.
  • - The research involved 31 necropsy cases from different types of flocks, investigating signs like increased mucus and hemorrhages in the respiratory tract, with notable findings of syncytia and viral inclusion bodies.
  • - Immunohistochemistry (IHC) successfully detected gallid alphaherpesvirus 1 (GaHV-1) in many tissues, indicating its effectiveness in supporting ILT diagnosis, especially when histopathological findings were unclear.
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Infectious bronchitis virus (IBV) is a gammacoronavirus that primarily induces an upper respiratory disease in chickens, also affecting the urogenital tract and occasionally leading to a condition called false layer syndrome (FLS), where sexually mature hens ovulate normally but are unable to lay eggs. Here, we describe an outbreak of FLS in Arizona from which an IBV variant that is nearly 90% homologous to DMV/1639 using the Spike subunit 1 gene, named , was isolated and used in challenge experiments. Three-day-old specific-pathogen-free chicks were challenged with AZ/FLS/17 or M41 in high and low doses, and the disease outcomes were compared.

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One dead 6-wk-old male racing pigeon () was submitted for postmortem evaluation after presenting with weight loss, anorexia, dry shanks, dehydration, and lethargy. The bird belonged to a confined flock with 12 other pigeons raised by a hobbyist. Two pigeons in the flock reportedly had died with a history of similar clinical signs.

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is an opportunistic Gram-positive bacterium that induces purulent lesions and abscesses in cattle, small ruminants, and swine. In birds, infections have been linked to lameness and osteomyelitis in turkeys (Phasianidae) and hepatic fibriscess in turkeys and pigeons (Columbidae). An 18-mo-old backyard rooster with a history of progressive emaciation was submitted to the California Animal Health and Food Safety (CAHFS) laboratory system.

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Augmentation of wild populations with captive-bred individuals presents an inherent risk of co-introducing novel pathogens to naïve species, but it can be an important tool for supplementing small or declining populations. Game species used for human enterprise and recreation such as the ring-necked pheasant (Phasianus colchicus) are commonly raised in captivity and released onto public and private wildlands as a method of augmenting naturalized pheasant populations. This study presents findings on pathogen exposure from three sources of serological data collected in California during 2014-2017 including (a) 71 pen-reared pheasants sampled across seven game bird breeding farms, (b) six previously released pen-reared pheasants captured at two study sites where wild pheasants occurred and (c) 79 wild pheasants captured across six study sites.

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One dead 6-week-old, male racing pigeon ( Columbia livia ) was submitted for postmortem evaluation after presenting with weight loss, anorexia, dry shanks, dehydration and lethargy. The bird belonged to a confined flock with 12 other pigeons raised by a hobbyist. Two pigeons in the flock reportedly had died with a history of similar clinical signs.

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Transmissible viral proventriculitis (TVP) is a disease of chickens, mostly in broilers of 2-8 wk of age. Chicken proventricular necrosis virus (CPNV), a birnavirus, is the etiologic agent. Characteristic gross lesions are enlargement, atony, and pallor of the proventriculus.

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Erysipelas is a bacterial disease caused by Erysipelothrix rhusiopathiae that affects multiple mammalian and avian species. In poultry, the disease is of sporadic prevalence and more often observed in older birds, leading to decreased egg production and mortality. Among avian species, turkey breeders seem to be the most affected, but outbreaks have been reported in ducks, layer chickens, quails, geese, and various captive and free-range birds.

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Article Synopsis
  • A 7-year-old backyard Leghorn chicken unexpectedly died and was submitted for postmortem examination.
  • During the examination, a hemorrhagic mass in the neck area was found, which upon closer inspection, showed characteristics of a neoplasm composed of epithelial cells and lymphocytes.
  • The lesion was classified as a thymoma based on specific microscopic features and immunohistochemistry tests, marking a rare diagnosis since only five cases have been recorded in birds at the facility from 1990 to 2019.
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In March 2019, the California Animal Health and Food Safety Laboratory (CAHFS), Turlock branch, received two submissions of broiler chickens from commercial flocks reporting increased mortality. Submissions consisted of either white or brown broilers. Submitted chickens appeared depressed with ruffled feathers.

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Necrotic enteritis (NE) is an important enteric disease affecting a wide variety of avian species, including poultry, caused by Clostridium perfringens type G and, rarely, type C. Significant economic losses can result from elevated mortality rates and poor performance, such as decreased weight gain associated with intestinal damage and impaired absorption of nutrients. Additional losses can result from elevated condemnation at the processing plant because of a high incidence of cholangiohepatitis.

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Article Synopsis
  • * In May 2018, a 1-year-old male Gouldian finch was diagnosed with the infection at a California lab, showing severe respiratory issues and high mortality rates among other finches in a mixed-species aviary.
  • * Investigations revealed significant liver and spleen damage, with detection of the chlamydia bacteria and possible circovirus particles in the liver, marking it as the first documented case of this dual infection in a Gouldian finch.
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The reemergence of infectious coryza (IC) caused by (AP) as an acute and occasionally chronic respiratory disease in domestic poultry has caused severe losses in several U.S. states.

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In 2017, the Turlock branch of the California Animal Health & Food Safety laboratory system received a significant increase in infectious coryza (IC) necropsy cases, with a total of 54 submissions originating from commercial broilers ( = 40), commercial layers ( = 11), and backyard chickens ( = 3). Layer flocks positive for IC were distributed within the adjacent counties of Merced and Stanislaus, while broiler flocks were concentrated within Merced County. The backyard flocks were located in Alameda and Sacramento counties.

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