Matrimeres are systemic nanoscale mediators of tissue integrity and function.

Tissue barriers must be rapidly restored after injury to promote regeneration. However, the mechanism behind this process is unclear, particularly in cases where the underlying extracellular matrix is still compromised. Here, we report the discovery of matrimeres as constitutive nanoscale mediators of tissue integrity and function.

Telestroke value through the eyes of emergency medicine providers: A counterfactual analysis.

Objectives: Emergency Medicine (EM) provider experiences consulting telestroke (TS) are poorly studied. In this qualitative study, we aimed to determine how TS changes patient management and to measure TS effects on EM provider confidence with acute ischemic stroke (AIS) treatment.

Materials And Methods: We designed a survey for EM providers querying perceptions of TS value, confidence with treating AIS, and counterfactuals regarding what EM providers would have done without TS.

Development of a programmable magnetic agitation device to maintain colloidal suspension of cells during microfluidic syringe pump perfusion.

Droplet-based microfluidic devices have been used to achieve homogeneous cell encapsulation, but cells sediment in a solution, leading to heterogeneous products. In this technical note, we describe automated and programmable agitation device to maintain colloidal suspensions of cells. We demonstrate that the agitation device can be interfaced with a syringe pump for microfluidic applications.

Predicting neuroimaging eligibility for extended-window endovascular thrombectomy.

Objective: Endovascular thrombectomy (EVT) and tissue plasminogen activator (tPA) are effective ischemic stroke treatments in the initial treatment window. In the extended treatment window, these treatments may offer benefit, but CT and MR perfusion may be necessary to determine patient eligibility. Many hospitals do not have access to advanced imaging tools or EVT capability, and further patient care would require transfer to a facility with these capabilities.

Effect of low recumbent angle on cycling performance, fatigue, and V˙O(2) kinetics.

Purpose: This study aimed to examine the effect of the degree of inclination from upright to supine postures on cycling performance, fatigue, and oxygen uptake (V˙O(2)) kinetics.

Methods: In experiment 1, 10 subjects performed graded and fatigue (exhaustive constant-load heavy exercise with 10 s all-out efforts interspersed every minute) tests at four cycling postures: upright, 30° recumbent (R), 15° R, and supine. In experiment 2, nine different subjects performed two bouts of constant-load heavy exercise in the same four cycling postures.

Process of discovery: a fourth-year translational science course.

Background: The Liaison Committee on Medical Education notes the importance of educating medical students on clinical and translational research principles.

Purpose: To describe a fourth-year course, "Process of discovery," which addresses teaching these principles, and to discuss students' perceptions of the course.

Methods: Core components and pedagogical methods of this course are presented.

IGF-I and microglia/macrophage proliferation in the ischemic mouse brain.

We have used a model of hypoxic-ischemic brain injury in adult male C57BL/6 mice to study insulin-like growth factor-I (IGF-I) and IGF-binding protein (IGFBP) expression in response to cerebral hypoxia-ischemia (H/I) in the adult mouse. A period of 20 min of H/I that resulted in histopathology in cortex, striatum, and thalamus was correlated with induction of mRNA for IGF-I, IGFBP-2, IGFBP-3, IGFBP-5, and glial fibrillary acidic protein (GFAP) by 4 days of recovery. Increased IGF-I mRNA was located within damaged regions and was surrounded by IGFBP-2 mRNA expression.