Publications by authors named "Steven Marsh"
Background: Patient ethnicity has been correlated with different outcomes after haematopoietic cell transplantation (HCT), with patients from minority ethnic backgrounds reported to have worse outcomes compared with White patients. To date, studies have been predominantly done in the USA, where health-care models are different to many European countries, including the UK. We aimed to evaluate the impact of patient-reported ethnicity on autologous and allogeneic HCT outcomes in the UK.
View Article and Find Full Text PDFEight novel HLA class I alleles have been identified using full gene sequencing.
View Article and Find Full Text PDFArticle Synopsis
- The IPD-MHC Database is a detailed and organized resource for non-human major histocompatibility complex (MHC) sequences, which helps in studying their function and evolution.
- The latest version features over 18,000 MHC alleles from 125 species, including a new section for cetaceans like whales and dolphins, all meticulously curated.
- Recent updates also introduce predicted tertiary structures for over 8,000 alleles and enhanced tools for comparing allele variations, facilitating advanced research in immunogenetics.
HLA antigens were historically defined according to the unique reactivity pattern of cells expressing HLA molecules with distinctive clusters of allo-antisera and/or monoclonal antibodies. Subsequently, amino acid residues determining epitopes (DEP) in the HLA molecule were correlated with reactivity patterns. In current clinical practice, the presence of allo-antibodies is assessed using Luminex-based solid phase single antigen bead (SAB) assays for transplantation.
View Article and Find Full Text PDFArticle Synopsis
- Strategies to enhance natural killer (NK) cell responses against cancer include using tumor-targeting antibodies, NK cell engagers, and adopting NK cells from healthy donors.
- KIR2DS2, an activating receptor on NK cells, is linked to better cancer outcomes in healthy individuals, but its optimal use in therapy is uncertain.
- Research found that KIR2DS2+ NK cells from cancer patients respond better to antibodies than KIR2DS2- cells, but the effectiveness of KIR2DS2+ NK cells from healthy donors decreases after expansion needed for therapeutic use.
Article Synopsis
- Researchers identified 43 new HLA alleles in blood cell donors through advanced DNA sequencing techniques.
- The use of single molecule real-time sequencing allowed for more accurate detection of these alleles.
- These findings can improve donor matching for transplants and enhance our understanding of genetic diversity in populations.
HLA typing: A review of methodologies and clinical impact on haematopoietic cell transplantation.
Best Pract Res Clin Haematol
June 2024
Article Synopsis
- The HLA gene system plays a crucial role in the success of hematopoietic cell transplants, influencing patient outcomes significantly.
- Advances in typing technologies have improved how HLA is identified, enhancing the resolution and detection of genetic variations within HLA loci.
- Current gold standard methods for HLA typing are designed to provide definitive results, reflecting the ongoing progress in this field.
Background: Simulations of physical processes and behavior can provide unique insights and understanding of real-world problems. Magnetic Resonance Imaging (MRI) is an imaging technique with several components of complexity. Several of these components have been characterized and simulated in the past.
View Article and Find Full Text PDFSequence confirmations of 175 HLA class I and II alleles in the IPD-IMGT/HLA Database.
View Article and Find Full Text PDFNomenclature for factors of the HLA system, update April, May and June 2024.
Int J Immunogenet
October 2024
Article Synopsis
- The IMGT/HLA Database, established in 1998 by the HLA Informatics Group, serves as a key repository for HLA allele variant sequences recognized by the WHO.
- The rise of next generation sequencing (NGS) has led to a significant increase in both the volume and complexity of data submitted to the database, enhancing HLA typing capabilities.
- To keep up with these advancements and the growing user base, the IPD-IMGT/HLA Database has had to innovate its data access and presentation methods while ensuring high standards of curation.
Optimizing natural killer (NK) cell alloreactivity could further improve outcome after allogeneic hematopoietic cell transplantation (alloHCT). The donor's Killer-cell Immunoglobulin-like Receptor (KIR) genotype may provide important information in this regard. In the past decade, different models have been proposed aiming at maximizing NK cell activation by activating KIR-ligand interactions or minimizing inhibitory KIR-ligand interactions.
View Article and Find Full Text PDFTwo novel alleles, HLA-G*01:04:09 and HLA-DPB1*04:01:01:136, were identified in a single healthy individual.
View Article and Find Full Text PDFTwo novel non-classical HLA class I alleles have been characterized, HLA-F*01:16 and -F*01:17.
View Article and Find Full Text PDFArticle Synopsis
- Optical Calorimetry (OC) uses interferometry to measure radiation-induced changes in refractive index, allowing direct measurement of absorbed doses in water.
- The study optimized an OC system for ultra-high dose rate applications, utilizing software for design and testing a prototype in a clinical setting.
- Key improvements included reducing noise, isolating the system from vibrations, and controlling temperature, leading to enhanced spatial resolution and lower dose uncertainty, making OC a promising method for advanced dosimetry in high dose rate treatments.