Late Manipulation under Anesthesia after Total Knee Arthroplasty: Improved Range of Motion and a Low Complication Rate.

The purpose of this study was to evaluate outcomes of late manipulation under anesthesia (MUA) for stiffness performed from ≥12 weeks to more than a year after primary total knee arthroplasty (TKA). A total of 152 patients receiving MUA ≥12 weeks after primary TKA from 2014 to 2021 were reviewed. The primary outcome measured was change in range of motion (ROM).

Cytotoxic and antimicrobial drimane meroterpenoids from a fungus of the (Ostropales, Ascomycota).

As part of our ongoing research on bioactive fungal metabolites, two new metabolites were isolated from a fungus of the (strain MSX62440), dasyscyphins F and G ( and ), and the known dasyscyphin C (). Compound was characterized by HRMS and 1D and 2D NMR data, and its absolute configuration established by ECD spectroscopy. A structural revision of dasyscyphin C () was based on NMR data and verified by ECD calculations.

Metabolites from the Marine-Facultative sp. MEXU 27854 and MEXU 29901 from Caleta Bay, Mexico.

During our ongoing research on fungal strains from unexplored sources, the reinvestigation of the CHCl-MeOH extract of the marine-facultative sp. MEXU 27854 yielded a new -methyl cyclic pentapeptide () along with known butyrolactone II and PF1233 A. In addition, from the marine-facultative MEXU 29901, a new alternariol glucoside, 10-[-D-(4-methoxyl-glucopyranosyl)]-4--methylalternariol () and known alternariol 4-methyl ether, alternariol and beauvericin, were isolated.

Engineering Fluorine into Verticillins (Epipolythiodioxopiperazine Alkaloids) via Precursor-Directed Biosynthesis.

Precursor-directed biosynthesis was used to generate a series of fluorinated verticillins. The biosynthesis of these epipolythiodioxopiperazine alkaloids was monitored via the droplet liquid microjunction surface sampling probe (droplet probe), and a suite of NMR and mass spectrometry data were used for their characterization. All analogues demonstrated nanomolar IC values vs a panel of cancer cell lines.

Structurally Modified Cyclopenta[]benzofuran Analogues Isolated from .

Four new cyclopenta[]benzofuran derivatives based on an unprecedented carbon skeleton (-), with a dihydrofuran ring fused to dioxanyl and aryl rings, along with a new structural analogue () of 5‴-episilvestrol (episilvestrol, ), were isolated from an aqueous extract of a large-scale re-collection of the roots of collected in Vietnam. Compound demonstrated mutarotation in solution due to the presence of a hydroxy group at C-2‴, leading to the isolation of a racemic mixture, despite being purified on a chiral-phase HPLC column. Silvestrol () and episilvestrol () were isolated from the most potently cytotoxic chloroform subfraction of the roots.

Meroterpenoids from Neosetophoma sp.: A Dioxa[4.3.3]propellane Ring System, Potent Cytotoxicity, and Prolific Expression.

Six fungal metabolites, of which five were new, including one (1) with a dioxa[4.3.3]propellane ring system, were discovered, identified, and structurally elucidated from Neosetophoma sp.