Publications by authors named "Steven E Nelson"

Reproduction is a physiologically demanding process for sea turtles. Health indicators, including morphometric indices and blood analytes, provide insight into overall health, physiology and organ function for breeding sea turtles as a way to assess population-level effects. The Archie Carr National Wildlife Refuge (ACNWR) on Florida's central eastern coast is critical nesting habitat for loggerhead sea turtles (), but health variables from this location have not been documented.

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Loggerhead sea turtles (Caretta caretta) are opportunistic carnivores that feed primarily on benthic invertebrates and fish. Sea turtle rehabilitation requires provision of a species-specific, balanced diet that supplies nutrition similar to that of a wild diet; this can be challenging because free-ranging loggerheads' diets vary depending on their life stage and geographic location, with predominant prey species dictated by local availability. The goal of this study was to better understand the nutritional needs of subadult and adult loggerheads in rehabilitation.

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Critically endangered black rhinoceros (BR) under human care are predisposed to non-hemochromatosis Iron Overload Disorder (IOD). Over the last 30 years, BR have been documented with diseases that have either been induced by or exacerbated by IOD, prompting significant efforts to investigate and address this disorder. IOD is a multi-factorial chronic disease process requiring an evidence-based and integrative long-term approach.

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The introduction of rabies virus (RABV) to barrier islands, which are often popular tourist destinations with resource-rich habitats and connectivity and proximity to the mainland, is especially concerning because it can easily become endemic due to factors like dense rabies-vector populations (e.g., raccoons [ Procyon lotor]), high inter- and intraspecies contact rates, and anthropogenic activities such as supplemental feeding of feral cats ( Felis catus).

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Objective: A range of doses of supplemental vitamin D has been shown to be effective in preventing rickets in breastfed infants, but the effect of different doses of vitamin D on bone metabolism and mineral content has not been delineated.

Methods: In a randomized trial, breastfed infants received from 2 to 9 months daily supplements of vitamin D in doses of 200 IU/d, 400 IU/d, 600 IU/d or 800 IU/d. Measures of bone metabolism (plasma) were determined periodically and bone mineral content (DXA) was determined at study entry and at the end of winter when infants were 5.

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Objectives: Infant formulas provide more protein than breast milk. High protein intakes may place infants at risk of later obesity. The present study tested whether a formula with protein content below the regulatory level supports normal growth from age 3 months.

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The objective of this study was to determine the pharmacokinetics of two orally administered doses of tramadol (5 and 10 mg/kg) and its major metabolite (O-desmethyltramadol) (M1) in loggerhead sea turtles (Caretta caretta). After oral administration, the half-life of tramadol administered at 5 and 10 mg/kg was 20.35 and 22.

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Article Synopsis
  • The study aimed to find the optimal timing for collecting blood samples to measure baseline corticosterone levels in loggerhead sea turtles while minimizing stress-related effects.
  • Significant increases in white blood cell counts were observed over time for both nesting and rehabilitating turtles, but no direct correlation with corticosterone levels was noted.
  • For nesting turtles, sampling within 3 minutes and for rehabilitating turtles within 6 minutes after handling is recommended to obtain accurate baseline corticosterone measurements.
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Marine turtle fibropapillomatosis (FP) is a debilitating, infectious neoplastic disease that has reached epizootic proportions in several tropical and subtropical populations of green turtles (Chelonia mydas). FP represents an important health concern in sea turtle rehabilitation facilities. The objectives of this study were to describe the observed epidemiology, biology, and survival rates of turtles affected by FP (FP+ turtles) in a rehabilitation environment; to evaluate clinical parameters as predictors of survival in affected rehabilitating turtles; and to provide information about case progression scenarios and potential outcomes for FP+ sea turtle patients.

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Background: Breastfed infants require supplementation with vitamin D (vD), but little is known about the necessary dose. This double blind trial evaluated four different doses of vD.

Methods: Exclusively breastfed infants (N = 213) were randomized at 1 mo to one of four doses, which they received through 9 mo while receiving no formula.

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The birth iron endowment provides iron for growth in the first months of life. We describe the iron endowment under conditions of low dietary iron supply. Subjects were infants participating in a trial of Vitamin D supplementation from 1 to 9 months.

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Objectives: Infant formulas provide more protein than breast milk. High protein intakes, as well as maternal obesity, are risk factors for later obesity. The present study tested whether a formula with lower protein content slows weight gain of infants of overweight mothers (body mass index [BMI] >25 kg/m).

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  • The 32-kDa movement protein P3 from alfalfa mosaic virus is crucial for the virus's movement between plant cells.
  • In tests with Nicotiana tabacum, P3 mainly appeared in cell walls of older leaves and in the cytosol of younger ones, while in Pichia pastoris, it was found primarily in a soluble state.
  • Metabolic labeling showed that P3 underwent phosphorylation in both organisms, indicating that this modification likely influences its function like in other viral movement proteins.
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  • Appropriate growth for premature infants means achieving growth without negative effects, which is crucial for cognitive development.
  • Nutritional management focuses on providing sufficient nutrient intake, starting with parenteral nutrition right after birth and using human milk for gut maturation; however, human milk must be fortified due to its incomplete nutrient profile.
  • Recent efforts at the authors' institution have led to better nutrient intakes, especially protein, resulting in improved growth outcomes and only mild postnatal growth failure in infants.
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Purpose Of Review: To describe the creation of the World Health Organization Child Growth Standards (WHO standards) released in 2006, to show their main features and to compare them with existing charts.

Recent Findings: The WHO standards are the first globally representative growth standards. They describe the growth of children worldwide who are living in favorable circumstances.

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  • Three studies aimed at improving iron status in breastfed infants were conducted.
  • These studies involved infants who were exclusively breastfed for the first 4 months and later received either iron supplements or fortified foods.
  • The findings showed that medicinal iron and fortified cereals effectively prevented iron deficiency (ID) and iron deficiency anemia (IDA) during the supplement period, but improvements were not sustained after stopping the interventions.
  • Overall, the research suggests that it's feasible to protect breastfed infants from ID and IDA with appropriate iron supplementation strategies.
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  • The study compared two infant cereals fortified with different forms of iron—electrolytic iron and ferrous fumarate—to evaluate their effectiveness in maintaining iron levels in breast-fed infants.
  • Both cereals were consumed by infants from 4 to 9 months of age, but no significant differences in iron status were found between the two groups during the study.
  • While a small percentage of infants showed mild iron deficiency, none developed iron deficiency anemia, suggesting that both types of iron fortification were equally effective.
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Growth charts compared.

Nestle Nutr Workshop Ser Pediatr Program

June 2010

Growth assessment of children requires comparison of growth measurements with normative references, usually in the form of growth charts. Traditionally growth charts (growth references) have described the growth of children who were considered normal and were living in a defined geographic area. The new WHO growth charts, on the other hand, are growth standards that aim to represent growth as it occurs worldwide.

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  • Breastfed infants can experience iron deficiency (ID), which may be prevented by providing iron regularly.
  • A study tested the effects of medicinal iron and iron-fortified cereal on breastfed infants' iron status over several months to see if it improved without negative side effects.
  • Both iron sources were effective in enhancing iron status, though medicinal iron was linked to slightly slower growth, indicating it might have trade-offs despite improving iron levels.
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Background: In breastfed infants, iron deficiency at <6 mo of life, although uncommon, is observed in industrialized countries. Iron supplementation starting at an early age may prevent iron deficiency.

Objective: The study assessed the effect of early iron supplementation of breastfed infants and tested the hypothesis that iron supplementation enhances iron status.

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  • The study examined vitamin D levels in breastfed infants in Iowa, finding that 10% were deficient at 280 days old, with higher rates in dark-skinned and unsupplemented infants during winter.
  • Longitudinal data indicated that unsupplemented infants were frequently vitamin D deficient, especially in winter, and severe deficiencies were linked to elevated parathyroid hormone and alkaline phosphatase levels.
  • The research concluded that vitamin D deficiency is prevalent among breastfed infants without supplementation, affecting infants of all skin tones, and recommends that all breastfed infants receive vitamin D supplements to prevent deficiency.
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  • Critically ill premature infants have high iron needs due to iron loss from blood draws, making iron absorption studies crucial for their care.
  • A study involving infants under 1300 g at birth examined the effects of intravenous sucrose iron combined with erythropoietin (EPO) on iron absorption and red blood cell (RBC) production, compared to control and EPO-only groups.
  • Results showed that the combination treatment significantly improved RBC iron incorporation and erythropoiesis but did not enhance iron absorption compared to EPO alone.
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Background: Successful prevention of obesity and related cardiovascular risk factors requires a clear understanding of its determinants over the life course. Rapid infancy weight gain is associated with childhood obesity, whereas low infancy weight is associated with coronary heart disease. Our aim was to identify during which periods in infancy weight gain is associated with adult obesity.

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For estimating the requirements for dietary iron, it is important to know the amount of iron that is lost from the body. Inevitable losses of iron have been determined in adult humans but not in infants or children. We administered (58)Fe, the least abundant stable isotope of iron, to free-living infants at 168 d of age (5.

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  • In growing adolescents, iron needs are determined by both growth requirements and the unavoidable loss of iron.
  • The study used a stable isotope of iron, (58)Fe, to track total body iron changes over a 1.56 to 3.29-year period, allowing researchers to estimate iron loss.
  • The findings showed that male adolescents lose approximately 256 mg of iron yearly (9.7% per year), while females lose about 306 mg yearly (14.6% per year), leading to estimated daily iron requirements of 1.46 mg for males and 1.15 mg for females, including both growth and loss.
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