Structural insights of two novel N-acetyl-glucosaminidase enzymes through in silico methods.

EndoBI-1 and EndoBI-2 are two endo- acetylglucosaminidase isoenzymes that cleave diacetylchitobiosyl moieties found in various types of native -glycans. These -glycans are indigestible by human infants and adults due to the lack of responsible glycosyl hydrolases and they act as selective prebiotics for a probiotic microorganism, subsp , in the large intestine. The selectivity and the thermostability of EndoBI-1 and EndoBI-2 suggest that these enzymes may be useful for many scientific and industrial applications.