Publications by authors named "Steve Lonsdale"
In 2022, the World Health Organization declared the worldwide outbreak of mpox to be a public health emergency of international concern. The causative monkeypox virus (MPXV) belonged to clade IIb and is transmitted through sexual contact with a low case fatality rate (0.1%), which, together with under-detection, all contributed to a rapid global spread particularly within the MSM (men who have sex with men) community.
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- Rapid inactivation of the Ebola virus (EBOV) is essential for effective testing in low-resource outbreak situations.
- Testing revealed that Buffer AVL (commonly used for RNA extraction) was ineffective in inactivating EBOV in 67% of samples, indicating it shouldn't be relied upon for virus inactivation in diagnostics.
- However, combining Buffer AVL with ethanol or heat treatments resulted in complete viral inactivation in all tested samples, while still allowing for the extraction of high-quality RNA for PCR analysis.