Impact of Predation by Ciliate on Conjugation in subsp. .

Background/objectives: Antibiotic resistance gene (ARG) spread is driven by horizontal gene transfer (HGT). Ciliated protozoa may contribute to this process, as their predation has been shown to facilitate HGT in certain bacteria. Here, this phenomenon was further investigated using subsp.

Whole-genome-based taxonomy as the most accurate approach to identify Flavobacterium species.

The genus Flavobacterium comprises a diversity of species, including fish pathogens. Multiple techniques have been used to identify isolates of this genus, such as phenotyping, polymerase chain reaction genotyping, and in silico whole-genome taxonomy. In this study, we demonstrate that whole-genome-based taxonomy, using average nucleotide identity and molecular phylogeny, is the most accurate approach for Flavobacterium species.

Specific amino acid changes correlate with pathogenic flavobacteria.

is a genus of microorganisms living in a variety of hosts and habitats across the globe. Some species are found in fish organs, and only a few, such as and , cause severe disease and losses in fish farms. The evolution of flavobacteria that are pathogenic to fish is unknown, and the protein changes accountable for the selection of their colonization to fish have yet to be determined.

Centroid of the bacterial growth curves: a metric to assess phage efficiency.

Phage replication can be studied using various approaches, including measuring the optical density (OD) of a bacterial culture in a liquid medium in the presence of phages. A few quantitative methods are available to measure and compare the efficiency of phages by using a single index based on the analysis of OD curves. However, these methods are not always applicable to non-canonical OD curves.

Year-round monitoring of three water sources in Québec, Canada, reveals site-specific differences in conditions for and contamination.

and are protozoan parasites responsible for gastrointestinal illnesses in humans and in animal species. The main way these parasites are transmitted is by ingestion of their (oo)cysts in drinking water. Monitoring (oo)cysts in water sources is beneficial to evaluate the quality of raw water supplying treatment plants.

Complete sequences of two sp. strains and one strain isolated from the environment on STAA medium highlight biotechnological potential.

Streptomycin thallous acetate actidione medium is typically used to isolate bacteria from food. Using this medium, three bacterial strains were isolated from the environment. Genomic sequences demonstrated that these bacteria are of the genera and and are of biotechnological interest.

Interaction of pAsa5 and pAsa8 Plasmids in subsp. .

The plasmid known as pAsa5 is present in subsp. , a fish pathogen. The pAsa5 plasmid carries genes that are essential for the bacterium's virulence.

displays high transformation capacity with complete cloning vector integration into the chromosomal plasticity zone.

The obligate intracellular genus contains many pathogens with a negative impact on global health and economy. Despite recent progress, there is still a lack of genetic tools limiting our understanding of these complex bacteria. This study provides new insights into genetic manipulation of with the opportunistic porcine pathogen , the only chlamydial species naturally harboring an antibiotic resistance gene, originally obtained by horizontal gene transfer.

Draft genome sequences of four strains, SC302, SC304, SC306, and SC310, isolated from swine from Eastern Canada.

The bacterium causes porcine exudative epidermitis in piglets, which represents both health and welfare concerns. Few genome sequences of this pathogen are published. We provide four additional ones to help future genomic analysis of .

MQM1, a bacteriophage infecting strains of Aeromonas salmonicida subspecies salmonicida carrying Prophage 3.

Aeromonas salmonicida subsp. salmonicida is a Gam-negative bacterium responsible for furunculosis in fish. Because this aquatic bacterial pathogen has a rich reservoir of antibiotic-resistant genes, it is essential to investigate antibacterial alternatives, including the use of phages.

: Genomics, Taxonomy, Diversity, Pathogenesis, Treatments and Beyond.

For a long time, the bacterial species seemed to be limited to a regrouping of psychrophilic subspecies that infect fish, particularly salmonids [...

Amino acid substitutions in specific proteins correlate with farnesol unresponsiveness in Candida albicans.

Background: The quorum-sensing molecule farnesol, in opportunistic yeast Candida albicans, modulates its dimorphic switch between yeast and hyphal forms, and biofilm formation. Although there is an increasing interest in farnesol as a potential antifungal drug, the molecular mechanism by which C. albicans responds to this molecule is still not fully understood.

Host Dependent-Transposon for a Plasmid Found in subsp. That Bears a Gene for Chloramphenicol Resistance.

Plasmids that carry antibiotic resistance genes occur frequently in subsp. , an aquatic pathogen with severe consequences in salmonid farming. Here, we describe a 67 kb plasmid found in the subsp.

isolates from Canada demonstrate wide distribution and clustering among mesophilic strains.

All the 36 known species to date of the genus are mesophilic except the species , which includes both psychrophilic and mesophilic subspecies. For 20 years, more and more mesophilic strains have been discovered. Only subsp.

Isolation of vB_AsaM_LPM4 reveals the dynamics of Prophage 3 in Aeromonas salmonicida subsp. salmonicida.

Aeromonas salmonicida subsp. salmonicida causes furunculosis, a major infection that affects fish farms worldwide. We isolated phage vB_AsaM_LPM4 (LPM4) from a diseased fish.

Expansion of the pRAS3 Plasmid Family in subsp. and Growing Evidence of Interspecies Connections for These Plasmids.

subsp. is a pathogenic bacterium responsible for furunculosis in salmonids. Following an outbreak of furunculosis, the infection can be treated with antibiotics, but it is common to observe ineffective treatment due to antibiotic resistance.

Antimicrobial Resistance in the Environment: Towards Elucidating the Roles of Bioaerosols in Transmission and Detection of Antibacterial Resistance Genes.

Antimicrobial resistance (AMR) is continuing to grow across the world. Though often thought of as a mostly public health issue, AMR is also a major agricultural and environmental problem. As such, many researchers refer to it as the preeminent One Health issue.

Aeromonas salmonicida intraspecies divergence revealed by the various strategies displayed when grazed by Tetrahymena pyriformis.

Worldwide, Aeromonas salmonicida is a major bacterial pathogen of fish in both marine and freshwater environments. Despite psychrophilic growth being common for this species, the number of characterized mesophilic strains is increasing. Thus, this species may serve as a model for the study of intraspecies lifestyle diversity.

Working toward improved monitoring of Cryptosporidium and Giardia (oo)cysts in water samples: testing alternatives to elution and immunomagnetic separation from USEPA Method 1623.1.

Objective: This study was designed to find a method to enhance the recovery of Cryptosporidium spp. and Giardia spp. parasites from water samples for research purposes compared to the results that can be achieved with USEPA Method 1623.

Detection of spp. and spp. in Environmental Water Samples: A Journey into the Past and New Perspectives.

Among the major issues linked with producing safe water for consumption is the presence of the parasitic protozoa spp. and spp. Since they are both responsible for gastrointestinal illnesses that can be waterborne, their monitoring is crucial, especially in water sources feeding treatment plants.

To Be or Not to Be Mesophilic, That Is the Question for .

The bacterium has long been known to be one of the most feared pathogens in fish farming. However, the more we discover about this bacterial species, the more we question whether it is really exclusively an aquatic pathogen. In recent years, it has become obvious that this bacterial species includes a myriad of strains with various lifestyle and ecological niches, including the well-known strict psychrophiles, the first bacteria known of the species, and the newly described mesophilic strains.

Comparative Genomics of Typical and Atypical Complete Genomes Revealed New Insights into Pathogenesis Evolution.

is a global distributed Gram-negative teleost pathogen, affecting mainly salmonids in fresh and marine environments. strains are classified as typical or atypical depending on their origin of isolation and phenotype. Five subspecies have been described, where   subsp.

Characterization of the Antibacterial Activity of an SiO Nanoparticular Coating to Prevent Bacterial Contamination in Blood Products.

Technological innovations and quality control processes within blood supply organizations have significantly improved blood safety for both donors and recipients. Nevertheless, the risk of transfusion-transmitted infection remains non-negligible. Applying a nanoparticular, antibacterial coating at the surface of medical devices is a promising strategy to prevent the spread of infections.

Phage Cocktail Development against subsp. Strains Is Compromised by a Prophage.

Aquaculture is a rapidly growing food production sector. Fish farmers are experiencing increasing problems with antibiotic resistance when fighting against pathogenic bacteria such as subsp. , the causative agent of furunculosis.