Publications by authors named "Steve Bolin"
The exquisite sensitivity of in vitro amplification assays such as real-time polymerase chain reaction (rtPCR) requires the establishment of thorough and robust laboratory practices. To this end, an American Association of Veterinary Laboratory Diagnosticians (AAVLD) committee of subject matter experts was convened to develop a set of best practices for performance of nucleic acid amplification assays. Consensus advice for the performance of preanalytical, analytical, and postanalytical steps is presented here, along with a review of supporting literature.
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- A study in Michigan aimed to see if Virginia opossums contribute to the persistence of Mycobacterium bovis infections in local animal populations.
- Researchers used 12 opossums, inoculating four with M. bovis while others were exposed or served as controls, then observed them for 45 days.
- The results showed that only the inoculated opossums tested positive for the infection, indicating no significant transmission of M. bovis occurred between infected and uninfected opossums during the study period.
Cutaneous toxoplasmosis has been previously reported in human beings, rarely reported in cats, and reported in 1 dog with systemic toxoplasmosis. The present report describes 2 cases of cutaneous toxoplasmosis in 2 dogs treated with immunosuppressive therapy. One of the dogs developed generalized cutaneous pustules and pruritus, and the other dog only had a single subcutaneous nodule.
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