Pulmonary vein isolation plus adjunctive therapy for the treatment of atrial fibrillation: a systematic review and meta-analysis.

Background: Pulmonary vein isolation (PVI) is the primary technique for ablation of atrial fibrillation (AF). It is unclear whether adjunctive therapies in addition to PVI can reduce atrial arrhythmia recurrence (AAR) compared to PVI alone in patients with AF.

Methods: A meta-analysis of randomized controlled trials comparing PVI plus an adjunctive therapy (autonomic modulation, linear ablation, non-pulmonary vein trigger ablation, epicardial PVI [hybrid ablation], or left atrial substrate modification) to PVI alone was conducted.

Translocation of cationic amphipathic peptides across the membranes of pure phospholipid giant vesicles.

The ability of amphipathic polypeptides with substantial net positive charges to translocate across lipid membranes is a fundamental problem in physical biochemistry. These peptides should not passively cross the bilayer nonpolar region, but they do. Here we present a method to measure peptide translocation and test it on three representative membrane-active peptides.

Statistical analysis of peptide-induced graded and all-or-none fluxes in giant vesicles.

Antimicrobial, cytolytic, and cell-penetrating peptides induce pores or perturbations in phospholipid membranes that result in fluxes of dyes into or out of lipid vesicles. Here we examine the fluxes induced by four of these membrane-active peptides in giant unilamellar vesicles. The type of flux is determined from the modality of the distributions of vesicles as a function of their dye content using the statistical Hartigan dip test.

Phase separation and fluctuations in mixtures of a saturated and an unsaturated phospholipid.

We describe quantitatively the interactions in a mixture of a saturated and an unsaturated phospholipid, and their consequences to the phase behavior at macroscopic and microscopic levels. This type of lipid-lipid interaction is fundamental in determining the organization and physical behavior of biological membranes. Mixtures of dipalmitoylphosphatidylcholine (DPPC) and 1-palmitoyl-2-oleoylphosphatidylcholine (POPC) are examined in detail by multiple experimental approaches (differential scanning calorimetry (DSC), fluorescence resonance energy transfer, and confocal fluorescence microscopy) in combination with Monte Carlo simulations in a lattice.