Prestained and Preloaded DMEK Grafts: An Evaluation of Tissue Quality and Stain Retention.

Purpose: To examine endothelial cell damage and stain retention of prestained preloaded Descemet membrane endothelial keratoplasty (DMEK) grafts.

Methods: DMEK grafts were stained with trypan blue or left unstained before loading into a Straiko modified Jones tube. A protocol to stain preloaded grafts inside the modified Jones tube and resulting cell loss was also examined.