Publications by Stephen B Lyle

Publications by authors named "Stephen B Lyle"

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Expression, purification, and characterization of peptidyl-tRNA hydrolase from Staphylococcus aureus.

Paul D Bonin Gil H Choi Catherine M Trepod John E Mott Stephen B Lyle

Protein Expr Purif

February 2002

Bacterial peptidyl-tRNA hydrolase (Pth) activity ensures the rapid recycling of peptidyl-tRNAs that result from premature termination of translation. Pth has been shown to be essential for growth in Escherichia coli suggesting that its homologue in Staphylococcus aureus is a potential molecular therapeutic target for the development of antibacterial agents. In this report we describe the cloning of a DNA fragment (573 bp) containing the pth gene from a S.

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