Publications by authors named "Stephanie Wisner"
Rewired metabolism is a hallmark of pancreatic ductal adenocarcinomas (PDA). Previously, we demonstrated that PDA cells enhance glycosylation precursor biogenesis through the hexosamine biosynthetic pathway (HBP) via activation of the rate limiting enzyme, glutamine-fructose 6-phosphate amidotransferase 1 (GFAT1). Here, we genetically ablated GFAT1 in human PDA cell lines, which completely blocked proliferation in vitro and led to cell death.
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- Cancer cells adapt their metabolism for survival under stress, and targeting these adaptations is a key research focus.
- The study examines how the inhibition of a specific enzyme, GOT1, in pancreatic cancer affects the cells' metabolic dependencies and redox balance.
- Findings show that blocking GOT1 leads to vulnerabilities in antioxidant functions and triggers ferroptosis, a type of cell death linked to iron and oxidative stress, highlighting a connection between GOT1, iron regulation, and cell death mechanisms.
Ras proteins play vital roles in numerous biological processes and Ras mutations are found in many human tumors. Understanding how Ras proteins are regulated is important for elucidating cell signaling pathways and identifying new targets for treating human diseases. Here we report that one of the K-Ras splice variants, K-Ras4a, is subject to lysine fatty acylation, a previously under-studied protein post-translational modification.
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