Postnatal transcriptional activity regulates functional properties of PV interneurons.

The transcription factor Aristaless-related X-linked gene () is a monogenic factor in early onset epileptic encephalopathies (EOEEs) and a fundamental regulator of early stages of brain development. However, expression persists in mature GABAergic neurons with an unknown role. To address this issue, we generated a conditional knockout (CKO) mouse in which postnatal was ablated in parvalbumin interneurons (PVIs).

Normal Development of the Perineuronal Net in Humans; In Patients with and without Epilepsy.

The perineuronal net (PN), a highly organized extracellular matrix structure, is believed to play an important role in synaptic function, including maturation and stabilization. In addition to its role in restricting plasticity, alterations in the PN are implicated in disorders such as epilepsy and schizophrenia. However, the time course of PN development is not known in humans.

A viral strategy for targeting and manipulating interneurons across vertebrate species.

A fundamental impediment to understanding the brain is the availability of inexpensive and robust methods for targeting and manipulating specific neuronal populations. The need to overcome this barrier is pressing because there are considerable anatomical, physiological, cognitive and behavioral differences between mice and higher mammalian species in which it is difficult to specifically target and manipulate genetically defined functional cell types. In particular, it is unclear the degree to which insights from mouse models can shed light on the neural mechanisms that mediate cognitive functions in higher species, including humans.

Persistent decrease in multiple components of the perineuronal net following status epilepticus.

In the rodent model of temporal lobe epilepsy, there is extensive synaptic reorganization within the hippocampus following a single prolonged seizure event, after which animals eventually develop epilepsy. The perineuronal net (PN), a component of the neural extracellular matrix (ECM), primarily surrounds inhibitory interneurons and, under normal conditions, restricts synaptic reorganization. The objective of the current study was to explore the effects of status epilepticus (SE) on PNs in the adult hippocampus.

Method for quantifying patient expectations and early recovery after total knee arthroplasty.

Many components of a surgeon's total knee arthroplasty (TKA) treatment regimen affect the rate of recovery, such as patient selection, preoperative education, surgical technique, pain management, and postoperative rehabilitation. Therefore, accurate counseling requires that the surgeon quantifies patient expectations and early recovery of the treatment regimen with a method that minimizes interviewer bias. Preoperatively and 4 to 5 weeks after TKA, 285 patients (306 consecutive primary TKAs) responded to a survey consisting of customized questions, the Oxford score, the SF-12, and Knee Society scores on a handheld data acquisition device.