Distinguished properties of cells isolated from the dentin-pulp interface.

Background: Dental odontoblasts produce dentin mineralized matrix, trigger immune responses and act as sensory cells. The understanding of the mechanisms of these functions has been particularly restricted due to the lack of odontoblasts being cultivable in vitro. Because of the lack of specific markers to identify cells of the odontoblastic lineage, properties of the cells isolated from the dentin-pulp interface were compared to dental pulp cells, periodontal ligament cells, osteoblasts, skin fibroblasts, epithelial cells (A549) and HeLa in the present study.

Influence of zirconia and lithium disilicate tooth- or implant-supported crowns on wear of antagonistic and adjacent teeth.

Purpose: To investigate the influence of crown material (lithium-disilicate, 3Y-TZP zirconia) and abutment type (rigid implant, resin tooth with artificial periodontium) on wear performance of their antagonist teeth and adjacent teeth.

Materials And Methods: A mandibular left first molar (#36) with adjacent human teeth (mandibular left second premolar: #35, mandibular left second molar: #37) and antagonistic human teeth (maxillary left second premolar: #25, maxillary left first molar: #26, maxillary left second molar: #27) was prepared simulating a section of the jaw. Samples were made with extracted human molars (Reference), crowned implants (Implant), or crowned resin tooth analogues (Tooth).

Validating laboratory simulation with resin-based materials for temporary fixed denture prostheses - Results from clinical and laboratory trials.

The aim of the current study was to relate an artificial aging protocol for the analysis of dental materials to their clinical performance. 20 fixed interim restorations (crowns and fixed denture prostheses (FDPs)) fabricated from two commercially available resin-based composites (RBCs) and a previous clinical trial served as templates for the fabrication of duplicate restorations. Duplicates were subjected to artificial aging using thermal cycling and mechanical loading.

Performance of resin materials for temporary fixed denture prostheses.

This study investigated the handling properties and clinical performance of two commercially available resin materials with slight differences in filler composition for the fabrication of fixed interim restorations. In a dental university setting, patients requiring prosthetic treatment were supplied with fixed interim restorations fabricated from two commercially available resin materials. To clarify the handling properties of the resin materials, dentists and undergraduate students completed a questionnaire.

Influence of placement instruments on handling of dental composite materials.

Objective: Applicability and stickiness of dental composites are influential factors for the properties of those materials and so indirectly affect function, longevity and esthetics of composite restorations in the clinic. Thus, this in vitro study aimed for the influence of different placement instruments' diameter, geometries and coatings on the handling of uncured resin composite materials.

Methods: A survey about application technique of resin composites, placement instrument diameter, geometry and coating, and application temperature was answered by 55 German dentists in private practice.

In-vitro performance of CAD/CAM crowns with insufficient preparation design.

Purpose: To compare the debonding and fracture force of different CAD/CAM composite crowns with respect to the influence of water storage (0d vs. 90d/37 °C) and types of cementation (adhesive vs. self-adhesive).

Effect of Decontamination and Cleaning on the Shear Bond Strength of High Translucency Zirconia.

(1) Background: This study evaluated the bonding performance of high translucency zirconia after diverse surficial decontamination and cleaning procedures. (2) Methods: High translucency zirconia (Lava Esthetic) specimens (2.0 mm × 20 mm × 10 mm) were exposed to different surface treatments prior to bonding to CoCr cylinders (d = 5 mm, height = 3 mm).

Cell responses to cariogenic microorganisms and dental resin materials-Crosstalk at the dentin-pulp interface?

Objective: Resin monomers released from unpolymerized dental adhesives or composites and bacterial products like lipopolysaccharide (LPS) or lipoteichoic (LTA) are simultaneously present in specific applications following treatment of deep caries lesions. This review is focused on evidence concerning cell responses as a result of the interactions between adaptive mechanisms activated by resin monomers and signaling pathways of the immune response triggered by LPS or LTA originating from cariogenic microorganisms.

Methods: Current understanding of dental caries progression and pathways in eukaryotic cells in response to LPS stimulation in a clinical situation as well as cell reactions to oxidative stress caused by resin monomers is analyzed based on publications available through online databases.

Flavin-containing enzymes as a source of reactive oxygen species in HEMA-induced apoptosis.

Objective: Oxidative stress induced by compounds of dental composites like 2-hydroxyethyl methacrylate (HEMA) due to excess formation of reactive oxygen species (ROS) disturbs vital cell functions leading to apoptosis. The sources of ROS in cells exposed to resin monomers are unknown. The present study investigates functions of flavin-containing ROS and RNS (reactive nitrogen species) producing enzymes in cells exposed to HEMA.

Interaction between LPS and a dental resin monomer on cell viability in mouse macrophages.

Objective: Lipopolysaccharide (LPS) from cariogenic microorganisms and resin monomers like HEMA (2-hydroxyethyl methacrylate) included in dentin adhesive are present in a clinical situation in deep dentinal cavity preparations. Here, cell survival, expression of proteins related to redox homeostasis, and viability of mouse macrophages exposed to LPS and HEMA were analyzed with respect to the influence of oxidative stress.

Methods: Cell survival of RAW264.

Evaluation of cell responses toward adhesives with different photoinitiating systems.

Objectives: The photoinitiator diphenyl-(2,4,6-trimethylbenzoyl)phosphine oxide (TPO) is more reactive than a camphorquinone/amine (CQ) system, and TPO-based adhesives obtained a higher degree of conversion (DC) with fewer leached monomers. The hypothesis tested here is that a TPO-based adhesive is less toxic than a CQ-based adhesive.

Methods: A CQ-based adhesive (SBU-CQ) (Scotchbond Universal, 3M ESPE) and its experimental counterpart with TPO (SBU-TPO) were tested for cytotoxicity in human pulp-derived cells (tHPC).

Activation of the Nrf2-regulated antioxidant cell response inhibits HEMA-induced oxidative stress and supports cell viability.

Oxidative stress due to increased formation of reactive oxygen species (ROS) in target cells of dental resin monomers like 2-hydroxyethyl methacrylate (HEMA) is a major mechanism underlying the disturbance of vital cell functions including mineralization and differentiation, responses of the innate immune system, and the induction of cell death via apoptosis. Although a shift in the equilibrium between cell viability and apoptosis is related to the non-enzymatic antioxidant glutathione (GSH) in HEMA-exposed cells, the major mechanisms of adaptive antioxidant cell responses to maintain cellular redox homeostasis are still unknown. The present study provides insight into the induction of a communicating network of pathways under the control of the redox-sensitive transcription factor Nrf2, a major transcriptional activator of genes coding for enzymatic antioxidants.

2-Hydroxyethyl methacrylate-induced apoptosis through the ATM- and p53-dependent intrinsic mitochondrial pathway.

Resin monomers of dental composites like 2-hydroxyethyl methacrylate (HEMA) disturb cell functions including responses of the innate immune system, mineralization and differentiation of dental pulp-derived cells, or induce cell death via apoptosis. The induction of apoptosis is related to the availability of the antioxidant glutathione, although a detailed understanding of the signaling pathways is still unknown. The present study provides insight into the causal relationship between oxidative stress, oxidative DNA damage, and the specific signaling pathway leading to HEMA-induced apoptosis in RAW264.

A review of adaptive mechanisms in cell responses towards oxidative stress caused by dental resin monomers.

Dental composite resins are biomaterials commonly used to aesthetically restore the structure and function of teeth impaired by caries, erosion, or fracture. Residual monomers released from resin restorations as a result of incomplete polymerization processes interact with living oral tissues. Monomers like triethylene glycol dimethacrylate (TEGDMA) or 2-hydroxylethyl methacrylate (HEMA) are cytotoxic via apoptosis, induce genotoxic effects, and delay the cell cycle.

The influence of glutathione on redox regulation by antioxidant proteins and apoptosis in macrophages exposed to 2-hydroxyethyl methacrylate (HEMA).

Resin monomers like 2-hydroxyethyl methacrylate (HEMA) disturb cell functions including responses of the innate immune system, mineralization and differentiation, or induce cell death via apoptosis. These phenomena are associated with oxidative stress and a reduction in the concentration of the antioxidant glutathione (GSH), resulting in imbalanced redox homeostasis. Thus far, the precise mechanism of how resin monomers interfere with cellular redox regulation is unknown.

Function of MAPK and downstream transcription factors in monomer-induced apoptosis.

The resin monomer triethylene glycol dimethacrylate (TEGDMA) disrupts vital cell functions, and the production of oxidative stress is considered a common underlying mechanism. The precise signaling pathways, however, that initiate monomer-induced effects, which disturb responses of the innate immune system, inhibit dentin mineralization processes, or induce apoptosis in target cells in vitro are still unknown. The present study provides insight into the causal relationship between TEGDMA-induced apoptosis and the activation of MAPK and transcription factors downstream using pharmacological inhibitors of the ERK1/2, p38 and JNK pathways.

Microleakage of silorane- and methacrylate-based class V composite restorations.

The marginal integrity of class V restorations in a silorane- and a group of methacrylate-based composite resins with varying viscosities was tested in the present study. Different adhesives (OptiBond FL, KerrHawe; AdheSE One, Vivadent; or Silorane System Adhesive, 3M ESPE) were applied to 168 standardized class V cavities. The cavities (n = 12) were filled with a wide range of different viscous composite resins: Filtek Silorane, 3M ESPE; els and els flow, Saremco; Tetric EvoCeram and Tetric EvoFlow, Vivadent; Grandio, Voco; and Ultraseal XT Plus, Ultradent.

Oxidative stress and cytotoxicity generated by dental composites in human pulp cells.

Dental composites are a source of residual monomers that are released into the oral environment. Since monomers act on cultured cells through reactive oxygen species (ROS), we hypothesized that composites generate ROS associated with cytotoxicity. Human pulp-derived cells were exposed to extracts of methacrylate-based materials including triethylene glycol dimethacrylate and 2-hydroxyethyl methacrylate-free composites (Tetric Ceram, Tetric EvoCeram, els, els flow, Solitaire 2) and a silorane-based composite (Hermes III).

Activation of stress-regulated transcription factors by triethylene glycol dimethacrylate monomer.

Triethylene glycol dimethacrylate (TEGDMA) is a resin monomer available for short exposure scenarios of oral tissues due to incomplete polymerization processes of dental composite materials. The generation of reactive oxygen species (ROS) in the presence of resin monomers is discussed as a common mechanism underlying cellular reactions as diverse as disturbed responses of the innate immune system, inhibition of dentin mineralization processes, genotoxicity and a delayed cell cycle. Yet, the signaling pathway through a network of proteins that finally initiates the execution of monomer-induced specific cell responses is unknown so far.

Resin monomer-induced differential activation of MAP kinases and apoptosis in mouse macrophages and human pulp cells.

Triethylene glycol dimethacrylate (TEGDMA) is a resin monomer which is released from polymerized dental composite materials. It induced apoptosis in various target cells or inhibition of LPS-induced cytokine production in cells of the immune system after prolonged exposure. In these tissues, mitogen-activated protein kinases (MAPK) regulate signal transduction pathways that support cell survival and cytokine synthesis.

Influence of TEGDMA on the mammalian cell cycle in comparison with chemotherapeutic agents.

Objectives: The dental resin monomer triethylene glycol dimethacrylate (TEGDMA) caused a cell cycle arrest in response to DNA damage. However, the underlying mechanisms are unclear. Therefore, the influence of TEGDMA on the cell cycle was analyzed in comparison with the chemotherapeutic agents adriamycin and mitomycin C (MMC), which arrest the cell cycle through different mechanisms.

Ceramic inlays and partial ceramic crowns: influence of remaining cusp wall thickness on the marginal integrity and enamel crack formation in vitro.

No information is currently available about what the critical cavity wall thickness is and its influence upon 1) the marginal integrity of ceramic inlays (CI) and partial ceramic crowns (PCC) and 2) the crack formation of dental tissues. This in vitro study of CI and PCC tested the effects of different remaining cusp wall thicknesses on marginal integrity and enamel crack formation. CI (n = 25) and PCC (n = 26) preparations were performed in extracted human molars.

Influence of different cusp coverage methods for the extension of ceramic inlays on marginal integrity and enamel crack formation in vitro.

No information is available to date about cusp design of thin (1.0 mm) non-functional cusps and its influence upon (1) marginal integrity of ceramic inlays (CI) and partial ceramic crowns (PCC) and (2) crack formation of dental tissues. The aim of this in vitro study was to investigate the effect of cusp coverage of thin non-functional cusps on marginal integrity and enamel crack formation.

Bond strength of adhesive systems to dentin and enamel--human vs. bovine primary teeth in vitro.

Objectives: This in vitro study compared the bonding performance of four adhesive luting agents to dentin and enamel of human and bovine primary teeth, in order to evaluate the suitability of primary bovine hard tissues for replacement of those of human origin for bond testing.

Methods: A composite (Clearfil AP-X) was bonded to specimens from 167 extracted human (n=88) and bovine (n=88) primary teeth using the following adhesive systems: Syntac Assortment (SY), Adaper Prompt L-Pop (PLP), iBond Gluma inside (IB) and Clearfil Protect Bond (PB). After 24h storage in distilled water, shear bond strength was determined according to ISO/TS 11405:2003.