Publications by authors named "Stephanie G S de Almeida"
Investigation of cytokine polymorphisms on viral infections after renal transplantation exhibit association between IFN-γ +874 A > T and CMV manifestations.
Int J Immunogenet
December 2022
Article Synopsis
- The study examined how specific genetic variations (polymorphisms) in TNF-α, IFN-γ, and IL-10 affect the likelihood of viral infections following kidney transplants, specifically looking at viruses like CMV, BKPyV, HHV-6, and EBV.
- Results showed that the IFN-γ+874 A > T variant, which leads to lower production of IFN-γ, was linked to a higher incidence of CMV disease and graft failure in patients receiving mycophenolate treatment.
- The findings highlight the significant impact of the IFN-γ+874 SNP on the risk of CMV infection in renal transplant recipients.
Costs may hinder the implementation of BK polyomavirus (BKV)-DNAemia screening in resource-limited kidney transplant (KT) centers. We analyzed data from two studies to assess the performance and potential cost saving of a dual-step screening strategy based on the use of a preliminary qualitative semi-nested PCR (snPCR) assay followed by BKV-DNAemia quantification after KT. In the preliminary study, in which 130 samples from 33 KT recipients were screened for BKV-DNAemia, the estimated positive and negative predictive values of snPCR, as compared to quantitative PCR (qPCR), were 88% and 99%, respectively.
View Article and Find Full Text PDFBKV and JCV belong to the Polyomaviridae family and are opportunistic agents associated with complications in immunocompromised individuals. Although a single screening assay for both viruses would be convenient, the diversity of BKV and JCV serotypes and genotypes is a methodological challenge. In this paper, we developed a PCR method able to detect and segregate BKV and JCV, despite these genetic discrepancies.
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