Publications by authors named "Stephanie G S Almeida"
Investigation of cytokine polymorphisms on viral infections after renal transplantation exhibit association between IFN-γ +874 A > T and CMV manifestations.
Int J Immunogenet
December 2022
Article Synopsis
- The study examined how specific genetic variations (polymorphisms) in TNF-α, IFN-γ, and IL-10 affect the likelihood of viral infections following kidney transplants, specifically looking at viruses like CMV, BKPyV, HHV-6, and EBV.
- Results showed that the IFN-γ+874 A > T variant, which leads to lower production of IFN-γ, was linked to a higher incidence of CMV disease and graft failure in patients receiving mycophenolate treatment.
- The findings highlight the significant impact of the IFN-γ+874 SNP on the risk of CMV infection in renal transplant recipients.
Costs may hinder the implementation of BK polyomavirus (BKV)-DNAemia screening in resource-limited kidney transplant (KT) centers. We analyzed data from two studies to assess the performance and potential cost saving of a dual-step screening strategy based on the use of a preliminary qualitative semi-nested PCR (snPCR) assay followed by BKV-DNAemia quantification after KT. In the preliminary study, in which 130 samples from 33 KT recipients were screened for BKV-DNAemia, the estimated positive and negative predictive values of snPCR, as compared to quantitative PCR (qPCR), were 88% and 99%, respectively.
View Article and Find Full Text PDFBKV and JCV belong to the Polyomaviridae family and are opportunistic agents associated with complications in immunocompromised individuals. Although a single screening assay for both viruses would be convenient, the diversity of BKV and JCV serotypes and genotypes is a methodological challenge. In this paper, we developed a PCR method able to detect and segregate BKV and JCV, despite these genetic discrepancies.
View Article and Find Full Text PDFThe aim of this study was to investigate the association of EBV and HPV with gingivitis and/or periodontitis according to the immunologic status. To this end, 74 oral biopsies from transplanted and non-transplanted individuals with the abovementioned oral manifestations were submitted to a screening by PCR for both viruses. According to the results, EBV was strongly associated with gingivitis and/or periodontitis in transplanted individuals (p = 0.
View Article and Find Full Text PDF