Publications by authors named "Stephanie D Schumacher"
J Biotechnol
October 2012
Article Synopsis
- Cytochrome P450 enzymes are important for various biochemical reactions and are significant in biotechnology and pharmaceutical applications, but they often require specific environments to function.
- Researchers expressed the CYP106A2 enzyme from Bacillus megaterium on the outer membrane of E. coli using a technique called Autodisplay, which allowed them to perform tests confirming the enzyme's surface exposure and activity.
- The study found that CYP106A2 could function effectively on E. coli cells, showing that the presence of the heme group (essential for the enzyme's activity) could be provided externally, especially in strains lacking the TolC protein, helping to demonstrate the potential for using Autodisplay to enhance enzyme functionality in industrial applications.
J Biotechnol
October 2012
Article Synopsis
- The cytochrome P450 enzyme system contains various enzymes that facilitate many chemical reactions, making them highly versatile catalysts; CYP3A4 is a key enzyme in this group that helps metabolize drugs and toxins.
- Expressing CYP3A4 in bacterial hosts is challenging due to its need for a membrane environment and limited substrate access, prompting researchers to explore alternative methods.
- In this study, CYP3A4 was successfully expressed on the outer membrane of Escherichia coli using Autodisplay, confirmed by techniques like SDS-PAGE and flow cytometry, demonstrating its catalytic activity without needing an external heme group.
