High-Density Guide RNA Tiling and Machine Learning for Designing CRISPR Interference in sp. PCC 7002.

While CRISPRi was previously established in sp. PCC 7002 (hereafter 7002), the design principles for guide RNA (gRNA) effectiveness remain largely unknown. Here, 76 strains of 7002 were constructed with gRNAs targeting three reporter systems to evaluate features that impact gRNA efficiency.