Publications by authors named "Stephania Contreras Castillo"
Stem cell decoupling underlies impaired lymphoid development during aging.
Proc Natl Acad Sci U S A
May 2023
Mammalian aging is associated with multiple defects of hematopoiesis, most prominently with the impaired development of T and B lymphocytes. This defect is thought to originate in hematopoietic stem cells (HSCs) of the bone marrow, specifically due to the age-dependent accumulation of HSCs with preferential megakaryocytic and/or myeloid potential ("myeloid bias"). Here, we tested this notion using inducible genetic labeling and tracing of HSCs in unmanipulated animals.
View Article and Find Full Text PDFCyclosporine (CsA) and tacrolimus (TAC) are immunosuppressant drugs characterized by a narrow therapeutic range and high pharmacokinetic variability. The effect of polymorphisms in genes related to the metabolism and transport of these drugs, namely , , and genes, has been evaluated in diverse populations. However, the impact of these polymorphisms on drug disposition is not well established in Latin American populations.
View Article and Find Full Text PDFArticle Synopsis
- Hydroxycarbamide (HC) is a drug known for its ability to induce cell cycle blockade and is primarily used for treating myeloproliferative neoplasms (MPNs).
- Recent studies indicate that HC also affects gene expression by influencing transcription factors and altering DNA methylation, beyond just halting cell division.
- Research involving both murine models and MPN patients reveals that HC not only restores normal gene expression in hematopoietic stem cells but also modifies DNA methylation patterns associated with key regulators of blood cell formation.
Article Synopsis
- Alternative splicing (AS) and alternative polyadenylation (APA) create diverse transcripts in mammals, influenced by epigenetic marks like H3K36me3 and DNA methylation during development and differentiation.* -
- The study reveals that H3K36me3 is not a key regulator of AS or APA, while identifying over 4000 host genes with intragenic CpG islands (iCGIs). These iCGIs show dynamic transcriptional activity depending on tissue and developmental stages.* -
- It highlights that iCGI transcription affects host gene transcription and pre-mRNA processing more significantly than H3K36me3 or DNA methylation, driving diverse transcript and protein expression across different times and