Integrating a novel concept of sentence optotypes into the RADNER Reading Charts.

Purpose: To add a new set of 24 sentence optotypes to the German version of the RADNER reading charts and to investigate whether sentences constructed based upon an optimised concept of sentence optotypes can be used together with the original 38 sentences.

Methods: Twenty-eight optimised sentence optotypes were constructed based upon the concept of sentence optotypes as established for the RADNER Reading Charts, with words having the same number of characters and syllables being placed in the same positions. The best comparable sentences were statistically selected in 30 volunteers.

A new principle for the standardization of long paragraphs for reading speed analysis.

Purpose: To investigate the reliability, validity, and statistical comparability of long paragraphs that were developed to be equivalent in construction and difficulty.

Methods: Seven long paragraphs were developed that were equal in syntax, morphology, and number and position of words (111), with the same number of syllables (179) and number of characters (660). For validity analyses, the paragraphs were compared with the mean reading speed of a set of seven sentence optotypes of the RADNER Reading Charts (mean of 7 × 14 = 98 words read).

Reading performance of monofocal pseudophakic patients with and without glasses under normal and dim light conditions.

Purpose: To evaluate reading performance of patients with monofocal intraocular lenses (IOLs) (Acrysof SN60WF) with or without reading glasses under bright and dim light conditions.

Setting: Austrian Academy of Ophthalmology, Vienna, Austria.

Design: Evaluation of a diagnostic test or technology.

Transient transfection coupled to baculovirus infection for rapid protein expression screening in insect cells.

Baculovirus infected insect cells are widely used for heterologous protein expression. Despite the power of this system, the use of baculovirus techniques for protein expression screening is hampered by the time and resources needed to generate each recombinant baculovirus. Here, we show that a transfection/infection based expression system is suitable for screening of expression constructs in insect cells and represents a valid alternative to other traditional screening methodologies using recombinant baculovirus.