Publications by authors named "Stephan Heynen"
Background: Activation of RNA-dependent stress kinase PKR, especially by viral double-stranded RNA, induces eukaryotic initiation factor 2 α-chain (eIF2α) phosphorylation, attenuating thereby translation. We report that this RNA-mediated negative control mechanism, considered a cornerstone of the cell's antiviral response, positively regulates splicing of a viral mRNA.
Results: Excision of the large human immunodeficiency virus (HIV) rev/tat intron depends strictly on activation of PKR by the viral RNA and on eIF2α phosphorylation.
Article Synopsis
- The rapid increase in DNA genome sequencing data has resulted in misannotations in public databases, particularly affecting next-generation sequencing methods.
- Recent entries like miR-1274b and miR-1274a in miRBase may originate from tRNA sequences due to shared identical stretches of nucleotides.
- The study suggests that further validation of these miRNAs is necessary, as evidence indicates their small RNA fragments likely come from tRNA processing rather than being true miRNAs.