Publications by authors named "Stepan Ovchinnikov"
Article Synopsis
- * The study introduces BacPROTACs, small-molecule degraders that efficiently target the ClpC:ClpP protease complex to initiate the degradation of specific microbial proteins.
- * These BacPROTACs not only activate ClpC but also demonstrate effective in vivo activity against mycobacteria, paving the way for antibiotic discovery and providing a versatile tool for studying bacterial protein degradation.
Article Synopsis
- Type II toxin-antitoxin (TA) systems are two-gene modules found in many prokaryotes, with GNAT toxins and DUF1778 antitoxins being key examples.
- GNAT toxins disrupt cell growth by interfering with translation through the acetylation of aminoacyl-tRNAs, primarily targeting Gly-tRNA isoacceptors.
- The study reveals that the ancestor of GNAT toxins likely had broader specificity, capable of acetylating multiple elongator tRNAs, highlighting significant evolutionary changes in substrate specificity over time.
Type II toxin-antitoxins systems are widespread in prokaryotic genomes. Typically, they comprise two proteins, a toxin, and an antitoxin, encoded by adjacent genes and forming a complex in which the enzymatic activity of the toxin is inhibited. Under stress conditions, the antitoxin is degraded liberating the active toxin.
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