Binding Stoichiometry of a Recombinant Selenophosphate Synthetase with One Synonymic Substitution E197D to a Fluorescent Nucleotide Analog of ATP, TNP-ATP.

The transformation of the strain DH5α (TM)-T1(R) with plasmid vector pET11a containing the cloned gene of bacterial selenophosphate synthetase (SPS), selD, from the E. coli BL21-Gold (DE3) strain gives an overproducing strain of SPS with one synonymic substitution, E197D. The transformation efficiency was estimated as 8 × 10(8) CFU/ μ g plasmid DNA.

[The use of a protoplast-generating method in selecting the lytic enzyme producer Streptomyces recifensis subsp. lyticus].

Optimal conditions for the formation of stable protoplasts of S. recifensis subsp. lyticus 2435 were defined and the use of the protoplasting procedure to increase the activity of the synthesis of extracellular enzymes was shown in principle possible.

[Intergeneric hydridization of the actinomycetes Streptoverticillium mycoheptinicum and Streptomyces coelicolor].

Intergeneric crossing of the actinomycetes Streptoverticillium mycoheptinicum and Streptomyces coelicolor yielded recombinants which were mostly nonviable and unstable despite a relatively high frequency (10(-2)-10(-4)) at which their colonies appeared. The rare viable and stable recombinants were prototrophs. The structure of antibiotics in the hybrid cell may be modified as follows from the differences in the antibiotic activity of the LIA-973 hybrid and the parent strains.