Suppression of HSF1 activity by wildtype p53 creates a driving force for p53 loss-of-heterozygosity.

The vast majority of human tumors with p53 mutations undergo loss of the remaining wildtype p53 allele (loss-of-heterozygosity, p53LOH). p53LOH has watershed significance in promoting tumor progression. However, driving forces for p53LOH are poorly understood.

Efficacy of USB microscopes for imaging forensic materials.

USB microscopy, a relatively new and developing technology, offers a highly portable and relatively inexpensive means for performing microscopy. However, it has not been established that USB microscopes are capable of imaging at a level deemed sufficient for forensic requirements. The purpose of this study was to determine the value, applicability, and limitations of current USB microscope technology by comparing USB microscopes with traditional microscopes by collecting images of common forensic trace materials.

Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy.

Differential interference contrast (DIC) microscopy is a powerful imaging tool that is most commonly employed for imaging microscale objects using visible-range light. The purpose of this protocol is to detail a proven method for preparing plasmonic nanoparticle samples and performing single particle spectroscopy on them with DIC microscopy. Several important steps must be followed carefully in order to perform repeatable spectroscopy experiments.

Atomically thin gallium layers from solid-melt exfoliation.

Among the large number of promising two-dimensional (2D) atomic layer crystals, true metallic layers are rare. Using combined theoretical and experimental approaches, we report on the stability and successful exfoliation of atomically thin "gallenene" sheets on a silicon substrate, which has two distinct atomic arrangements along crystallographic twin directions of the parent α-gallium. With a weak interface between solid and molten phases of gallium, a solid-melt interface exfoliation technique is developed to extract these layers.

Monitoring the Stimulated Uncapping Process of Gold-Capped Mesoporous Silica Nanoparticles.

To establish a new method for tracking the interaction of nanoparticles with chemical cleaving agents, we exploited the optical effects caused by attaching 5-10 nm gold nanoparticles with molecular linkers to large mesoporous silica nanoparticles (MSN). At low levels of gold loading onto MSN, the optical spectra resemble colloidal suspensions of gold. As the gold is removed, by cleaving agents, the MSN revert to the optical spectra typical of bare silica.

FOXA1 expression is a strong independent predictor of early PSA recurrence in ERG negative prostate cancers treated by radical prostatectomy.

FOXA1 (Fork-head box protein A1, HNF-3a) is a transcription factor involved in androgen signaling with relevance for lineage-specific gene expression of the prostate. The expression was analyzed by immunohistochemistry on a tissue microarray containing 11152 prostate cancer specimens. Results were compared with tumor phenotype, biochemical recurrence, androgen receptor expression, ETS-related gene (ERG) status and other recurrent genomic alterations.

Heterogeneity of ERG expression in prostate cancer: a large section mapping study of entire prostatectomy specimens from 125 patients.

Background: TMPRSS2:ERG fusions are frequent in prostate cancer, and occur predominantly in young patients. Several studies had proposed intratumoral heterogeneity of these fusions. This study was designed to determine frequency and extent of ERG fusion heterogeneity in early-onset prostate cancer (EO-PCA, <50 years) and in elderly patients.

Detecting plasmon resonance energy transfer with differential interference contrast microscopy.

Gold nanoparticles are ideal probes for studying intracellular environments and energy transfer mechanisms due to their plasmonic properties. Plasmon resonance energy transfer (PRET) relies on a plasmonic nanoparticle to donate energy to a nearby resonant acceptor molecule, a process which can be observed due to the plasmonic quenching of the donor nanoparticle. In this study, a gold nanosphere was used as the plasmonic donor, while the metalloprotein cytochrome c was used as the acceptor molecule.

Influence of polarization setting on gold nanorod signal at nonplasmonic wavelengths under differential interference contrast microscopy.

Researchers rely on a variety of microscopic techniques for observing and tracking anisotropic nanoparticles in real time experiments. This technical note focuses on the optical behavior exhibited by gold nanorods at nonplasmonic wavelengths under differential interference contrast microscopy (DIC). Intense diffraction patterns appear at nonplasmonic wavelengths, and the behavior of these patterns can be altered by adjusting the surrounding medium or the polarizer setting.

Influence of gold nanorod geometry on optical response.

As noble metal nanoparticles are deployed into increasingly sophisticated environments, it is necessary to fully develop our understanding of nanoparticle behavior and the corresponding instrument responses. In this paper, we report on the optical response of three important gold nanorod configurations under dark field and differential interference contrast (DIC) microscopy after first establishing their absolute geometries with transmission electron microscopy (TEM). The observed longitudinal plasmon wavelengths of single nanorods are located at wavelengths consistent with previously developed theory.

Optical imaging of non-fluorescent nanoparticle probes in live cells.

Precise imaging of cellular and subcellular structures and dynamic processes in live cells is crucial for fundamental research in life sciences and in medical applications. Non-fluorescent nanoparticles are an important type of optical probe used in live-cell imaging due to their photostability, large optical cross-sections, and low toxicity. Here, we provide an overview of recent developments in the optical imaging of non-fluorescent nanoparticle probes in live cells.

Specific immunotherapy with SQ standardized grass allergen tablets in asthmatics with rhinoconjunctivitis.

Background: The best way to prevent allergy symptoms is to treat the allergic condition. Specific immunotherapy with grass allergen tablets 75,000 SQ-T (Grazax, Phleum pratense, ALK-Abelló) is safe and efficacious in rhinoconjunctivitis patients. As rhinoconjunctivitis often co-exists with asthma, we aimed to confirm safety and efficacy in grass allergic subjects with asthma and rhinoconjunctivitis.

[Patients' evaluation of coercion in a psychiatric department. Interview studies in psychiatric departments for adult patients at the Hillerød hospital].

During an 11 month period, use of coercion was prospectively registered in a psychiatric department. Of all patients who had been subjected to coercion 36% were (n = 86) interviewed subsequently. Eleven percent of the interviewed patients did not know, that they had been submitted to coercion, 22% did not know the reason and 30% did not agree with the motivation for the decision.

[The use of coercion at psychiatric departments for adults, Hillerød hospital. A descriptive study of the period 10 January 1991-31 August 1992].

The study describes the type and amount of coercion used during 11 months of 1992. Four hundred and fourty-one cases were included. Young patients i.

[Compulsory admissions to Frederiksberg Hospital after introduction of the new psychiatric legislation compared with the period just before the change of the law].

The new Danish Act on confinement and other means of compulsion in psychiatry came into force October 1st, 1989. To investigate changes connected with the new act all involuntary commitments to the psychiatric department of the hospital of Frederiksberg, with a catchment area of 85,000 inhabitants, were prospectively registered during 23 months before, 25 months after the new legislation. Information about the commitment was obtained from the admission papers and the case records.

[Compulsory procedures at the Psychiatric Department, Frederiksberg Hospital, before and after introduction of the new psychiatric legislation. 3. Compulsory medication].

The purpose of this investigation was to register if the new Danish law concerning commitment and compulsory procedures in psychiatry from 1.10.1989 caused changes in the extent of forced medications.

[Use of restraints in a psychiatric department, Frederiksberg Hospital, before and after introduction of the new psychiatric law. Restraining devices].

The purpose of this investigation was to register if the new Danish law concerning commitment and compulsory procedures in psychiatry from 1.10.1989 caused changes in the use of restraints.

[Compulsory procedures in the psychiatric department, Frederiksberg Hospital, before and after introduction of the new psychiatric legislation].

Registration of compulsory procedures in a psychiatric department before and after the reform of the Danish law concerning commitment and compulsory procedures in psychiatry. The purpose of this investigation was to register if the law reform 01.10.

[Compulsory admissions at the Frederiksberg hospital during the period 1 November 1987 to 30 September 1989. 2. Patients' assessment and experiences of the admissions].

The object of this prospective, descriptive investigation was to illustrate how the committed patients assessed and experienced the actual compulsory commitment and their assessment of the Danish legislation concerning mental illness by means of an interview investigation on discharge. During the period of investigation of 23 months, there were 64 compulsory admissions. Of these, 31 were on account of danger (red admission papers) and 33 on health grounds (yellow admission papers).

[Compulsory admissions at the Frederiksberg hospital during the period 1 Nov 1987 to 30 Sep 1989. 1. Characteristics of the compulsory admissions and the admitted persons].

During a period of 23 months from 1 Nov. 1987 to 30 Sep. 1989, before the new Danish legislation came into force, there were 64 compulsory commitments to the Psychiatric Department in Frederiksberg Hospital.

Quantifying depressive symptomatology: inter-rater reliability and inter-item correlations.

In this study an evaluation of the inter-rater reliability of the Hamilton Depression Rating Scale, the Melancholia Scale and the Montgomery-Asberg Depression Rating Scale has been carried out. Furthermore, the inter-item correlations of these scales have been investigated in relation to diagnostic ratings. It was found that the quantitative scales had satisfactory inter-rater relationship.

[Increased use of resources at psychiatric departments after the introduction of legislation on limitation of liberty and other forms of restriction in psychiatry. A prospective 3-month study].

The Danish legislation on restriction of liberty and other forms of compulsion in psychiatry was introduced on 1.10.1989.