[General adaptation syndrome (Selye) in the calf. 5. Effect of increased glucocorticosteroid values on the phagocytosis activity of luekocytes, the function of RHS and the morphology of lymphatic organs].

Studies were conducted with the view to elucidating the correlations between increased glucocorticosteroid levels in the blood and the defense potential of calf organism against infectious diseases. The test animals were exposed to several substances (ACTH, cortisol, colibacteria, coliendotoxin), and even one to two days of increased 11-OHKS values were followed by marked decline in phagocytosis activity of leucocytes. In addition, RHS function was considerably reduced, after ten to thirteen days of application had elapsed, since at that point the disappearance of intravenously applied bacteria from circulating blood of test animals took place at rates which were much lower than those recorded from untreated calves.

Characterization of fluorescein isothiocyanate. II. Absorption and fluorescence after conjugation to human- and rabbit-gamma-globulin and bovine serum albumin.

The alteration of extinctions of both FITC isomers by coupling to bovine serum albumin, human, and rabbit-gamma-globulin were studied. The extinction coefficients of these FTC conjugates were established. The emission characteristics of the FITC isomers and their conjugates were investigated under conditions normaly used in fluorescence microscopy.